IDseek® Mitochondrial DNA HVR Sequencing by MPS Sample Kit
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IDseek® Mitochondrial DNA HVR Sequencing by MPS Kit
Reverse Complement (RC-PCR) based MPS library prep solution for analysis of hypervariable regions (mutation hotspots) in very low quantities of (highly degraded) nuclear mtDNA in complex samples that do not provide a full STR. For laboratories in those countries where full genome sequencing in forensics is restricted by guidelines or regulations.
RC-PCR provides multiplex targeted amplification, sequencing indexing and adaptor addition occuring simultaneously in a single closed tube reaction.
All amplicons are designed to meet the shortest possible fragment lengths and are compatible with illumina® MiSeq™ systems, with 2x 10 bp Unique Dual Index reads.
IDseek® Kits have been tested with Tannic Acid and Humic Acid. RC-PCR technology allows the reliable analysis of highly degraded samples frequently found in forensic casework.
IDseek® Reagents and Consumables
IDseek® comprises a complete solution of reagents and consumables required for generating sequence-ready MPS libraries for Forensic applications: from indexing to amplification, magnetic library clean-up and size selection.
IDseek® reagents and consumables required for 96 reactions:
- RC-PCR Probe Mix
- RC-PCR HiFi 2x Hotstart PCR Mastermix
- 2x Dehydrated Pre-spotted Unique Index Primer Plates
- AmpliClean™ Cleanup, Magnetic Beads
A magnet compatible with 1.5 mL/2.0 mL centrifuge tubes is recommended for use with the AmpliClean™ Magnetic Bead Cleanup.