His-tag Protein ELISA kit
Datasheet (PDF) | Safety Data Sheets (MSDS)(PDF)
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Description
A His-tag is an amino acid motif that contains at least six consecutive histidine (His) residues, often at the N- or C-terminus of a protein. More than 50% of recombinant proteins expressed by eukaryotic or prokaryotic expression systems are tagged with His-tag. Selective, sensitive and quantitative detection of protein levels and protein-protein binding partners expressed with His-tag is therefore a valuable tool in Life Science research. BioVision’s His-Tag Protein ELISA Kit is a competitive-based ELISA that is easier, faster and more sensitive method to detect His-tag proteins expressed in both bacterial, insect and mammalian cells. This detection kit offers ready-to-use reagents, and can detect as low as 20 ng/ml of His-tag protein within 90 min.
Datasheet
| Cat # +Size | E4550-100 |
|---|---|
| Size | 100 assays |
| Kit Summary | • Detection method- Absorbance (450 nm) • Species reactivity- Universal • Application- quantitative measurement of His-Tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Universal |
| Applications | This ELISA kit is used for quantitative measurement of His-Tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies |
| Features & Benefits | • This ELISA kit is used for in vitro quantitative determination of His-Tag Protein • Detection Range: 25.6 – 3200 ng/ml • Sensitivity: 20 ng/ml |
| Kit Components | • ELISA Microplate • Standard • HRP-conjugate • Antibody Stock • TMB substrate • Stop Solution • Sample Diluent • Antibody Diluent • Wash Buffer • Plate Sealers |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
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Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.
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