A His-tag is an amino acid motif that contains at least six consecutive histidine (His) residues, often at the N- or C-terminus of a protein. More than 50% of recombinant proteins expressed by eukaryotic or prokaryotic expression systems are tagged with His-tag. Selective, sensitive and quantitative detection of protein levels and protein-protein binding partners expressed with His-tag is therefore a valuable tool in Life Science research. BioVision’s His-Tag Protein ELISA Kit is a competitive-based ELISA that is easier, faster and more sensitive method to detect His-tag proteins expressed in both bacterial, insect and mammalian cells. This detection kit offers ready-to-use reagents, and can detect as low as 20 ng/ml of His-tag protein within 90 min.
|Cat # +Size||E4550-100|
|Kit Summary||• Detection method- Absorbance (450 nm) • Species reactivity- Universal • Application- quantitative measurement of His-Tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies|
|Detection Method||Absorbance (450 nm)|
|Applications||This ELISA kit is used for quantitative measurement of His-Tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies|
|Features & Benefits||• This ELISA kit is used for in vitro quantitative determination of His-Tag Protein • Detection Range: 25.6 – 3200 ng/ml • Sensitivity: 20 ng/ml|
|Kit Components||• ELISA Microplate • Standard • HRP-conjugate • Antibody Stock • TMB substrate • Stop Solution • Sample Diluent • Antibody Diluent • Wash Buffer • Plate Sealers|
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
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