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The Next Revolution in Microbial NGS!

Introduction:
All bacteria and archaea contain a ribosomal subunit known as 16S that is around 1,500 nucleotides long and contains nine hypervariable regions (V1-V9) spread out among highly conserved regions that are genus- or speciesspecific. Sequencing of the 16S ribosomal RNA (rRNA) gene, an established genetic marker, is the gold standard for the analysis of bacterial samples for identification and classification of both pure cultures and mixed sample analysis. Through introduction of Next-generation Sequencing (NGS), the 16S rRNA method is now also widely used to deconvolute complex microbial communities, such as soil samples and human gut microbiomes.

EasySeq™ 16S rRNA V3V4 Bacterial ID NGS Library Prep kit is designed to sequence the hypervariable regions V3 and V4 in a singleplex reaction (Table 2), using a single amplicon that is aimed at deconvoluting complex bacterial communities. It is compatible with Illumina® MiSeq and NextSeq 1000/2000 (2x250bp) runs, both suitable for 16S metagenomic sequencing, enabling microbiologists to evaluate bacterial diversity and detect the abundance of microbes in various environments.

Compatibility:
This kit is used with DNA extracted from pure culturesand also allow direct amplification of DNA from clinical materials. Libraries are compatible with Illumina®.

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