DNA/RNA Shield Fecal Collection Tube

R1101

Cat #NameSize
R1101DNA/RNA Shield-Fecal Collection Tube10 pack
Documents
Description

DNA/RNA Shield Fecal Collection tubes are designed for the collection and preservation of nucleic acids from stool specimens. These fecal collection tubes take a microbial snapshot of a sample while inactivating viruses making samples safe and ready for transport. Samples stored in the these fecal collection tubes are stable at ambient temperature, and can be frozen for longer-term storage. Simply collect a spoonful of the specimen into the stool collection tubes prefilled with preservative and shake vigorously to ensure proper stabilization.

Technical Specifications
Applicable ForNext-Generation Sequencing, qPCR, microarray
Device Specs20 x 76 mm screw cap tube prefilled with DNA/RNA Shield (9mL). Spoon attached to screwcap.
Device StorageRNA: Ambient temperature (4°C-25°C) > 1 month
DNA: Ambient temperature (4°C-25°C) > 2 years
DNA & RNA: Frozen (< -20°C): Indefinitely
Registration StatusCE-IVD certified
Sample Collection1g or 1mL stool sample
Sample SourceStool, soil, environmental samples

 

product faq


Q1: Can DNA/RNA Shield be used on extracted DNA and/or RNA?

Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).

Q2: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?

Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.

Q3: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?

No, tissues do not need to be homogenized.

Q4: How to collect solid tissues in DNA/RNA Shield?

Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.

Q5: Can I FACS sort directly into DNA/RNA Shield?

Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).

Q6: How long can samples be stored frozen in DNA/RNA Shield?

DNA & RNA (<-20°C): Indefinitely

Q7: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?

RNA > 1 month
DNA > 2 years

Q8: What sample types is DNA/RNA Shield suitable for?

DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).

Video


reviews


Courtney Victor

Emory University

Jun 02 2021

5 review stars

Great for field work and comparable with other stabilization buffers!

The sequencing and PCR results we received from our fecal samples collected in these tubes were of high quality and comparable with other nucleic acid preservation approaches. The best feature is the ability to store samples in ambient temperature, which makes field work in remote locations more feasible.

Application Area: Analyze the gut microbiome from stool samples


 

Greg Jacobson

University of Waikato

Jun 02 2020

5 review stars

Excellent storage solution for stool DNA.

I used these as a storage solution for microbiome study and was very impressed with the product. Recommended.

Application Area: Store DNA in stool without degradation


 

Greg Jacobson

University of Waikato

Nov 27 2019

5 review stars

Excellent, I find them great for the described application.

These are an easy to use and effective way of preserving bacterial DNA in biological samples.

Application Area: Store biological samples before DNA prep for microbiome analysis