DNA/RNA Shield Fecal Collection Tube
|DNA/RNA Shield-Fecal Collection Tube
|DNA/RNA Shield Fecal Collection Tube (with Beads)
DNA/RNA Shield Fecal Collection tubes are designed for the collection and preservation of nucleic acids from stool specimens. These fecal collection tubes take a microbial snapshot of a sample while inactivating viruses making samples safe and ready for transport. Samples stored in the these fecal collection tubes are stable at ambient temperature, and can be frozen for longer-term storage. Simply collect a spoonful of the specimen into the stool collection tubes prefilled with preservative and shake vigorously to ensure proper stabilization. R1137 is pre-filled with 5 glass beads to help facilitate homogenization
|Next-Generation Sequencing, qPCR, microarray
|R1101: 20 x 76 mm screw cap tube prefilled with DNA/RNA Shield (9mL). Spoon attached to screwcap.
R1137: 20 x 76 mm screw cap tube prefilled with DNA/RNA Shield (4mL) and 5 glass beads. Spoon attached to screwcap.
|RNA: Ambient temperature (4°C-25°C) > 1 month
DNA: Ambient temperature (4°C-25°C) > 2 years
DNA & RNA: Frozen (< -20°C): Indefinitely
|1g or 1mL stool sample
|Stool, soil, environmental samples
Q1: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
Q2: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.
Q3: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q4: How to collect solid tissues in DNA/RNA Shield?
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
Q5: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).
Q6: How long can samples be stored frozen in DNA/RNA Shield?
DNA & RNA (<-20°C): Indefinitely
Q7: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month
DNA > 2 years
Q8: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Jun 02 2021
Great for field work and comparable with other stabilization buffers!
The sequencing and PCR results we received from our fecal samples collected in these tubes were of high quality and comparable with other nucleic acid preservation approaches. The best feature is the ability to store samples in ambient temperature, which makes field work in remote locations more feasible.
Application Area: Analyze the gut microbiome from stool samples
University of Waikato
Jun 02 2020
Excellent storage solution for stool DNA.
I used these as a storage solution for microbiome study and was very impressed with the product. Recommended.
Application Area: Store DNA in stool without degradation
University of Waikato
Nov 27 2019
Excellent, I find them great for the described application.
These are an easy to use and effective way of preserving bacterial DNA in biological samples.
Application Area: Store biological samples before DNA prep for microbiome analysis