dGTP BrilliantDye™ Terminator (v1.1) Cycle Sequencing kit (100 rxn)

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dGTP BrilliantDye™ Terminator (v1.1) Cycle Sequencing kit (100 rxn)



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Catalog# BRDG1-100

Variants:100 rxn

Price Not Available



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Product Information



The dGTP BrilliantDye Cycle Sequencing Kit is optimized for sequencing through GT- and G-rich templates.

Format
The dGTP BrilliantDye Terminator Kit replaces dITP with dGTP, enabling you to read through difficult-to-sequence templates by reducing early signal loss.


Compatibility
Compatible with ABI genetic analyzers of the 310, 3100, 3130, 3500, 3730 and SeqStudio series. Can be mixed with BrilliantDye v1.1 for optimal results.



Premium features



Same high performance, but with greater value

✔ Based on the trusted Sanger Chain Termination method

✔ No need for changes in protocol, setting or calibration

✔ Compatible with all ABI genetic analyzers






Faqs



What can I do to sequence through a hairpin or secondairy structure?

– Use the BrilliantDye dGTP kit for the sequencing reaction. For optimal results it can be mixed with the default BrilliantDye kits to increase read lenghts. – Add DMSO to a final 5% (v/v) concentration to the sequencing reaction. – Incubate the reaction at 96C for 10 minutes before cycle sequencing.

How can I troubleshoot an issue with Sanger Sequencing?

-A control primer (M13) and template (pGEM) are provided with each BrilliantDye kit. These can be run following the BrilliantDye protocol and can help you determine whether failed reactions are caused by poor template quality or sequencing reaction failure.

What is the difference between the different versions of BrilliantDye?

-The BrilliantDye Terminator v1.1 Cycle sequencing kit is designed for specialty applications that require optimal basecalling adjacent to the primer. The BrilliantDye Terminator v3.1. Cycle sequencing kit is the method of choice for longest reads. For sequencing through G- and GT-rich templates, NimaGen offers the dGTP BrilliantDye kits, optimized for challenging sequence templates.





References



“Cost-perfomance relation is significantly better than that of the other vendors”

The Genetics Department decided to use NimaGen´s D-Pure™ DyeTerminator kit on the Microlab® STARlet in the future for the sequencing projects since its cost-perfomance relation is significantly better than that of the other vendors, with no compromise in data quality and recovery.

Department of Human Genetics, Radboud University Medical Center





Downloads



Sanger Sequencing product guide PDF
BrilliantDye brochure PDF
BrilliantDye v1.1 Protocol PDF







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dGTP BrilliantDye™ Terminator (v1.1) Cycle Sequencing kit (1000 rxn)e





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