Description
Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
Datasheet
| Antibody Target | C-Myc |
|---|---|
| Alternate Name | MYC , C-MYC , c-Myc |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Synthetic peptide corresponding to residues surrounding amino acid 413 of human c-Myc |
| Accession # | P01106 |
| Gene ID | 4609 |
| Appearance | Colorless liquid |
| Concentration | 0.5 mg/ml |
| Formulation | 100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Myc-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal. |
| Purification | Affinity purified |
| Species Reactivity | All species |
| Application | Western blot, Immunoprecipitation, Immunofluorescence |
| Application & Usage | Western blotting (1-2 µg/ml), immunoprecipitation (20-40 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect end |
| Handling | The antibody solution should be gently mixed before use. |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
FAQ
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CITATIONS
Kumar, G. R. et. al. Importin α-Mediated Nuclear Import of Cytoplasmic Poly(A) Binding Protein Occurs as a Direct Consequence of Cytoplasmic mRNA Depletion. Mol. Cell. Biol., Aug 2011; 31: 3113 - 3125.
Lei, X. et. al. Proteolytic Processing of Angiopoietin-like Protein 4 by Proprotein Convertases Modulates Its Inhibitory Effects on Lipoprotein Lipase Activity. J. Biol. Chem., May 2011; 286: 15747 - 15756.
Liu, J. et al. Angiopoietin-like Protein 3 Inhibits Lipoprotein Lipase Activity through Enhancing Its Cleavage by Proprotein Convertases. J. Biol. Chem., Sep 2010; 285: 27561 - 27570.
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