Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
|Alternate Name||MYC , C-MYC , c-Myc|
|Immunogen||Synthetic peptide corresponding to residues surrounding amino acid 413 of human c-Myc|
|Formulation||100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Myc-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Species Reactivity||All species|
|Application||Western blot, Immunoprecipitation, Immunofluorescence|
|Application & Usage||Western blotting (1-2 µg/ml), immunoprecipitation (20-40 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect end|
|Handling||The antibody solution should be gently mixed before use.|
|Storage Conditions||-20 °C|
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
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