KEY FEATURES:• HPDP contains a dithiopyridyl group which is reactive towards sulfhydryl groups (-SH) such as in cysteine (C) side chain to form stable disulfide bond• Contains a spacer arm with a length of 29.2 Å that prevents steric hindrance to biotin labeling• The spacer is cleavable at the disulfide bond with reducing agent, such as DTT, TCEP, etc.• Must be dissolved in an organic solvent such as DMF or DMSO before dilution with aqueous buffersAPPLICATIONS:• Ideal for biotinylating antibody or protein for use in protein detection, purification or immobilization
|SKU-Size|| 2329-50 (Size: 50 mg) |
2329-250 (Size: 250 mg)
|Alternate Name||N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)-propionamide; Biotin-HPDP, pyridyldithiol-biotin, pyridyldisulfide-biotin|
|Appearance||White to Off-white Solid|
|Purity||≥90% by NMR|
|Solubility||DMSO or DMF|
|Handling||Protect from moisture|
|Storage Conditions||4 ̊C|
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
The product is not dissolving well in DMSO. Can it be dissolved by heat?
To ensure complete dissolution of the product, gently warm the mixture to 37 °C and vortex or sonicate. The stock should be aliquoted and stored frozen.
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