Phase Separation Reagent is molecular biology grade 1–bromo–3–chloropropane (BCP).
Phase Separation Reagent is molecular biology grade 1–bromo–3–chloropropane (BCP). It replaces chloroform, the highly volatile compound commonly used for phase separation in the single-step method of total RNA isolation and in the TRI Reagent® method. Substituting BCP for chloroform moderately improves the quantity and quality of the isolated RNA (1).
1. Chomczynski, P. and Mackey, K. Substitution of chloroform by bromochloropropane in the single-step method of RNA isolation. Anal. Biochem. 225: 163-164. 1995.
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