BCA Protein Quantitation Kit


Catalog# K812

Size: 1000 assays


Price Not Available

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Description




The BCA Protein Assay Kit provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution. The method utilizes a copper (Cu²ᶧ) salt which can be reduced to the cuprous state by protein(s). The generated Cu²ᶧ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm. The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).


Datasheet




Cat # +Size K812-1000
Size 1000 assays
Detection Method Absorbance (562 nm)
Applications Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).
Features & Benefits • Simple procedure; takes ~ 30-90 minutes • Fast and convenient
Kit Components • BCA Reagent • Copper Reagent • BSA Standard (10 mg/ml)
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.




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CITATIONS

Badreldin H. Ali, Testicular Toxicity of Water Pipe Smoke Exposure in Mice and the Effect of Treatment with Nootkatone Thereon. Oxid Med Cell Longev, June 2019; 31341528.
Yeast Model of Barth Syndrome. J. Biol. Chem., Apr 2015; 290: 9284 - 9298.
Yu Chen, et. al. Leucine-stimulated mTOR signaling is partly attenuated in skeletal muscle of chronically uremic rats. Am J Physiol Endocrinol Metab, Nov 2011; 301: E873 - E881.










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