Description
Biovision’s BCA Protein Assay kit is useful for colorimetric detection and quantification of total protein content even in the presence of detergents. The BCA Protein Assay Kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline medium. The amount of purple-colored product formed during the reaction can be correlated to the amount of protein in the BCA protein assay. The Cu+1-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a wide range of protein concentrations between 25-2000 μg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. This BCA Protein Assay Kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of the samples.
Datasheet
| Cat # +Size | K813-2500 (2500 assays) K813-5000 (5000 assays) |
| Detection Method | Absorbance (562 nm) |
|---|---|
| Species Reactivity | N/A |
| Applications | N/A |
| Features & Benefits | • BCA Reagent A • BCA Reagent B • BSA Standard (2 mg/ml) |
| Kit Components | • BCA Reagent A • BCA Reagent B • BSA Standard (2 mg/ml) |
| Storage Conditions | RT |
| Shipping Conditions | RT |
| USAGE | For Research Use Only! Not For Use in Humans. |
FAQ
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Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.
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