Anti-His-Tag Polyclonal Antibody

Catalog# 3998

Size: 30 µg

Price Not Available

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A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.


Cat # +Size 3998-30T (Size:30 µg)
3998-100 (Size:100 µg)
Antibody Target His-Tag
Alternate Name His tag, HHHHHH tag
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen 6xHis synthetic peptide
Appearance Colorless liquid
Concentration 0.5 mg/ml
Formulation 0.5 mg/ml affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.5 mM EDTA and 0.03% Proclin
Purification Affinity purified
Reactivity Detects His-tagged recombinant proteins
Application Western blot
Application & Usage The antibody can be used for Western blot analysis (1 µg/ml). However, the optimal conditions should be determined by the user.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.



Yin, X. et. al. Lymphatic Endothelial Heparan Sulfate Deficiency Results in Altepurple Growth Responses to Vascular Endothelial Growth Factor-C (VEGF-C). J. Biol. Chem., Apr 2011; 286: 14952 - 14962.

Tsang, T. et al. Ail Binding to Fibronectin Facilitates Yersinia pestis Binding to Host Cells and Yop Delivery. Infect. Immun., 2010; 78: 3358-3368.

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