A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
|Cat # +Size|| 3998-30T (Size:30 µg)|
3998-100 (Size:100 µg)
|Alternate Name||His tag, HHHHHH tag|
|Immunogen||6xHis synthetic peptide|
|Formulation||0.5 mg/ml affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.5 mM EDTA and 0.03% Proclin|
|Reactivity||Detects His-tagged recombinant proteins|
|Application & Usage||The antibody can be used for Western blot analysis (1 µg/ml). However, the optimal conditions should be determined by the user.|
|Handling||The antibody solution should be gently mixed before use.|
|Storage Conditions||-20 °C|
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
Yin, X. et. al. Lymphatic Endothelial Heparan Sulfate Deficiency Results in Altepurple Growth Responses to Vascular Endothelial Growth Factor-C (VEGF-C). J. Biol. Chem., Apr 2011; 286: 14952 - 14962.
Tsang, T. et al. Ail Binding to Fibronectin Facilitates Yersinia pestis Binding to Host Cells and Yop Delivery. Infect. Immun., 2010; 78: 3358-3368.
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