BCA Protein Assay Kit (Test Tube) – Reducing Agent Compatible

BCA Protein Assay Kit (Test Tube) – Reducing Agent Compatible

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Catalog# K819

Size: 250 assays

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BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & β-mercaptoethanol up to 35 mM. This kit is adapted from BioVision’s BCA Assay Kit II (cat. # K813) and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is useful for determining protein concentrations of samples containing DTT, TCEP & β-mercaptoethanol. The assay is linear over the widest range of protein concentration between 25-2000 μg/ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.


Cat # +Size K819-250
Size 250 assays
Detection Method Absorbance (562 nm)
Applications Measuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Features & Benefits • Simple & convenient. • Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & β-mercaptoethanol (35 mM). • The assay is linear over a wide range of protein concentration (25-2000 μg/ml).
Kit Components • BCA Reagent A • BCA Reagent B • BSA Standard (2 mg/ml) • Blocking Reagent (20 mg/tube) • Blocking Reagent Buffer
Storage Conditions RT
Shipping Conditions RT
USAGE For Research Use Only! Not For Use in Humans.



Mohamed M. Elshemy. Antioxidative Capacity of Liver- and Adipose-Derived Mesenchymal Stem Cell-Conditioned Media and Their Applicability in Treatment of Type 2 Diabetic Rats. Oxid Med Cell Longev. Feb 2021. 33623636.

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