Predesigned and custom panels available targeting up to 100 amplicons in a single closed tube reaction.
EasySeq NGS Reverse Complement PCR
RC-PCR
Contamination Concerns?
Conventional Amplicon based methods for NGS target enrichment involve two PCR steps – one to amplify the regions of interest followed by a second PCR reaction to add adapter sequences for NGS. While these methods are well established there is always a high risk of cross contamination or carry-over from first to second rounds of amplification
Legal Notice:
RC-PCR is IP protected.
US Patent / WO Patent / EP Patent
Exclusive license granted to NimaGen B.V., Nijmegen, The Netherlands
by SALISBURY NHS FOUNDATION TRUST (SALISBURY, WILTSHIRE, GB)
It is strictly prohibited to use, produce, distribute, import, or sell RC-PCR based methods or kits without the patent owner’s written consent.
How it Works
Easy Handling
Just one Pre-PCR reaction setup, quick, easy and highly suited for automation
RC-PCR
Product generation is more target specific
Traditional PCR reactions have high concentrations of primer at the beginning of the reaction when the template availability is low.
In a RC-PCR the target specific primer is synthesized during the reaction and consequently more aligned with the target availability reducing the chance for dimerization and off target binding.
Multiplex Capabilities
Solutions available and in development:
- Human Sample tracking and ID kit for WGS/WES QC
- Forensic Identification Kits
- Crop Genotyping assays (GreenoType)
- GMO detection kit
- Meat detection kit (including speciate)
- Pharmacogenetics (PGX)
- Circulating Tumour DNA kit
- Detection of aneuploidies
