Homogenizer HOMEX

Homogenizer HOMEX

BIOREBA’s distinctive Homogenizing system is specifically tailored for the effective grinding of plant samples. We provide a semi-automated solution, the HOMEX 7, ideal for high-throughput applications. Additionally, our HOMOGENIZER handheld model is designed for efficiently grinding a smaller number of samples. For even greater efficiency, our recently introduced HOMEX XS is an automatic homogenizer capable of processing up to 100 samples per hour. Complementing these homogenizers, our reliable disposable extraction bags are perfectly compatible with the entire HOMEX product line.

HOMEX 7
The semi-automated HOMEX 7 is ideal for tissue extraction for samples to be used in ELISA and PCR. Samples are contained within our extraction bags throughout the sample processing, thereby eliminating cross-contamination and the need of washing between samples. Tissue samples of different thickness weighing up to several grams may be homogenized. About 250 samples per hour can be processed.
 
Bioreba HOMEX 7 can be equipped with two different racks to hold different sizes of extraction bags (see Product Information Extraction bags).
 
Standard rack (Cat. No. 400014S)
Bioreba HOMEX 7 is equipped with this standard rack. It holds 1- 5 extraction bags with a width of 12 cm and a length of 12-15 cm. Changing racks is just a matter of a few seconds.
 
Rack 4/25 (Cat. No. 4000164)
This rack holds 1-4 bags with a width of 15 cm and a length of 28 cm. The extraction bag is perfectly fixed when closing the rack. Herewith you avoid any leaking during the homogenization phase. Extraction bags 15 x 28 cm of a very strong quality with 15 mm sealing borders are available with a synthetic intermediate layer.
 
HOMEX XS
The HOMEX XS is an automated easy-to-use system for the homogenization of all kinds of tissues without sharp edges.
With the HOMEX XS the sample is homogenized and the filtered extract can then be used for analyses such as ELISA, PCR or other analytical applications.
 
It is an efficient, space saving machine with many advantages:
 
• High efficiency with homogenization of more than 100 samples per hour
• Optimized for BIOREBA extraction bags
• Adjustable rotation speed
• Adjustable homogenization time
• Stop timer function
• Ergonomic handling
 
Homogenizer Hand Model
The hand model is suitable for small sample numbers and for samples of softer tissue such as leaves, sprouts, flowers etc. The hand model can be used with all our extraction bags. For optimal grinding it is recommended to work on a hard surfac

Watch Video: Homogenizer HOMEX 7

Watch Video: Homogenizer HOMEX XS

Extraction Bags

Extraction Bags

Bioreba homogenization system comprises four distinct extraction bags, each available with or without a synthetic intermediate layer:

  1. Universal Extraction Bags: 12 x 15 cm
  2. U-Form Universal Extraction Bags: 12 x 15 cm
  3. Long Universal Extraction Bags: 15 x 28 cm
  4. Long Extraction Bags: 15 x 40 cm
  •  

BIOREBA’s extraction bags are well-suited for tissue extraction, specifically designed for use in ELISA and PCR applications.

Each unit comprises 100 bags. To purchase 1000 bags (a full box), simply place an order for the Extraction bag item 10 times.

The characteristics of BIOREBA extraction bags are:

– Optimal sampling and sample preparation (samples can be labeled and stored)
– Optimal homogenization
– Optimal filtration of the plant extracts (bags with synthetic intermediate layer)
– Optimal recovery of the plant sap
– No cross-contamination between test samples
– Optimal for homogenizing with our HOMEX system
– Suitable for homogenizing leaves, sprouts, roots, seeds, woody samples, sieved soil
 

AgriStrip

AgriStrip

The AgriStrip, a swift one-step assay created and produced by BIOREBA, relies on lateral flow immunochromatography technology. This AgriStrip test has been designed to verify the existence of a plant pathogen in samples displaying suspicious symptoms. It is conveniently offered in both a two-set and a single kit format.

AgriStrip Set

ComponentAssays
 25100
AgriStrips25 pcs.100 pcs.
AgriStrip Extraction buffer
ready-to-use
100 ml500 ml
AgriStrip Complete Kit
 
ComponentAssays
 25
AgriStrips25 pcs.
AgriStrip Extraction buffer
ready-to-use
100 ml
Disposable pipettes25 pcs.
Extraction bags25 pcs.
Disposable cuvettes25 pcs.
Cuvette rack,
holds 12 cuvettes
  1 pcs.
Optional Product
The homogenizer hand model is optional and not included in the “AgriStrip Complete Kit”.
 
ProductArt. No.Quantity
Homogenizer hand model400010    1
Extraction Bags “Universal”430100100
Cuvette rack, holds 12 cuvettes    2166    1
Cuvettes, disponible    2534100
Pipettes, disponible    2292500

Application

The performance of the test is very easy and there is no need for special laboratory equipment. Therefore, the AgriStrip is especially suitable when on-site testing is required, such as in greenhouses or fields.

Test Procedure and Results

A sample with suspicious symptoms is homogenized and a few drops of the homogenate are transferred to a cuvette.
The strip is dipped into the extract and the result can be read within a few minutes.
A positive result is seen as a colored band at the test line, whereas the colored band at the control line should always appear.
Test strips can be kept as permanent records.

Watch Video: AgriStrip Classic

Potato DNA/RNA Rapid Extraction

Potato DNA/RNA Rapid Extraction

The Potato DNA/RNA Rapid Extraction Kit is specifically designed for phytodiagnostic applications, streamlining the process of extracting DNA and RNA from potato tuber and leaf samples. This kit utilizes just two buffers, simplifying the extraction process and eliminating time-consuming steps. One noteworthy cost-saving benefit is that the Potato DNA/RNA Rapid Extraction Kit does not necessitate expensive machinery.

Advantages of BIOREBA Rapid Extraction
 
• Fast extraction in 30 minutes only
• Easy to use: only 5 steps
• Optimized for potato samples
• No specialised machines are needed
• Room temperature storage and shipment
• Total nucleic acids – extracts DNA and RNA in one procedure
• Validated and accredited method according to ISO / IEC 17025
• Extracted nucleic acids can be used for diagnostic tests such as real time PCR and PCR macroarray
• For pools of up to 25 potato samples
• Assays for viruses, bacteria and fungi
 

Watch Video: Potato Rapid Extraction.

PCR / qPCR Sets & Kits

PCR, qPCR Sets

  • ✔ Fast and reliable protocol
  • ✔ Test security due to lot-to-lot consistency
  • ✔ Cost effective 
  • ✔ Time saving

We provide a range of qPCR products that have undergone rigorous validation to ensure fast and dependable protocols. By utilizing our ready-to-use qPCR tests from BIOREBA, our clients can significantly reduce the time and effort required compared to creating their own laboratory solutions.

Bioreba qPCR products come with lot-specific data sheets, reflecting our commitment to maintaining consistent quality from one batch to another, thereby enhancing the reliability of your tests.

Bioreba comprehensive qPCR sets encompass essential components such as Taq Master Mix, RT Master Mix (designed for RNA virus analysis), Nuclease-free Water, as well as Primers, Probes, and Internal Control Mix.

In addition to Bioreba qPCR sets, our qPCR kits also include rapid DNA/RNA extraction solutions, offering a complete and efficient solution for your molecular biology needs.”

qPCR potato virus set & qPCR ToBRFV set

ComponentAssays
 96192
Taq Master Mix1.2 ml2 x 1.2 ml
RT Master Mix50 ul2 x 50 ul
Primers/Probes/IC Mix200 ul2 x 200 ul
Nuclease free water1 ml2 x 1 ml
 
qPCR grapevine virus set

 

ComponentAssays
 96192
Taq Probe Master Mix1.2 ml2 x 1.2 ml
Primers/Probes/IC Mix200 ul2 x 200 ul
Nuclease free water1 ml2 x 1 ml
 
qPCR potato virus kit

 

ComponentAssays
 96192
Taq Master Mix1.2 ml2 x 1.2 ml
RT Master Mix50 ul2 x 50 ul
Primers/Probes/IC Mix200 ul2 x 200 ul
Nuclease free water1 ml2 x 1 ml
Potato DNA/RNA rapid
extraction set*
up to 10 tuber
samples
up to 25 tuber
samples
 

 

ComponentAssays
 96192
Pool size: up to 10 tuber samples:
Rapid extraction buffer EB 1100 ml200 ml
Rapid extraction buffer EB 250 ml100 ml
Extraction bags Standard 12×15 cm100 pcs.2 x 100 pcs.
Pool size: up to 25 tuber samples:
Rapid extraction buffer EB 1200 ml2 x 200 ml
Rapid extraction buffer EB 250 ml100 ml
Extraction bags Standard 12×15 cm100 pcs.2 x 100 pcs.

Crops

Crops

BIOREBA provides diagnostic tests for plant pathogens in the following categories of crops:

  1. Potatoes
  2. Grapevines
  3. Vegetables
  4. Fruit Trees & Small Fruits
  5. Field Crops
  6. Ornamentals

Overview BIOREBA Plant Pathogen Diagnostic Tests

Crop:  AgriStrip            ELISA                  PCR, qPCR             PCR macroarray
Potatoesxxxx
Grapevines xx 
Vegetablesxxx 
Fruit Trees & Small Fruitsxxx 
Field Cropsxxx 
Ornamentalsxxx 

Elisa Reagents, Sets and Kits

ELISA

Enzyme-Linked Immunosorbent Assay (ELISA) is the assay format that is very popular in terms of efficiency, reproducibility, performance and validation in the plant pathogen diagnostic.

Bioreba ELISA reagents are delivered with Lot specific data sheets. Bioreba are committed to lot-to-lot consistency for increased test security.


IgG and Conjugate are available for 100, 500 and for 1000 assays.

Reagent                Assays   
 10050010005000*
IgG0.025 ml0.1 ml0.2 ml1 ml
Conjugate0.025 ml0.1 ml0.2 ml1 ml

*For the six main potato viruses (PLRV, PVA, PVM, PVS, PVX and PVY), reagents are also available for 5000 assays.

Control                    Assays
 12
Positive control2.5 ml
Negative control2.5 ml


Component Assays
480 960 5000*
IgG 0.1 ml 0.2 ml 1 ml
Conjugate 0.1 ml 0.2 ml 1 ml
Positive control 2.5 ml 2.5 ml 5 x 2.5 ml
Negative control 2.5 ml 2.5 ml 5 x 2.5 ml
Microtiter plates 5 10 60
*For the six main potato viruses (PLRV, PVA, PVM, PVS, PVX and PVY), reagents are also available for 5000 assays.


Component Assays
96 480 960 5000*
IgG 25 ul 0.1 ml 0.2 ml 1 ml
Conjugate 25 ul 0.1 ml 0.2 ml 1 ml
Positive control 2.5 ml 2.5 ml 2.5 ml 1 ml
Negative control 2.5 ml 2.5 ml 2.5 ml 5 x 2.5 ml
Extraction buffer (10x)
50 ml 250 ml 500 ml 5 x 500 ml
Coating buffer 1 tablet 1 tablet 2 tablets 10 tablets
Conjugate buffer (10x)
2 ml 10 ml 20 ml 100 ml
Substrate buffer (5x)
4 ml 20 ml 2 x 20 ml 200 ml
Washing buffer 1 tablet 1 pouch 2 pouches 2 containers
Substrate (pNPP)
4 tablets
(5 mg)
5 tablets
(20 mg)
10 tablets
(20 mg)
bulk powder
(5g)
Egg Albumin 5 g 25 g 50 g 250 g
Microtiter plates 1 5 10 60
*For the six main potato viruses (PLRV, PVA, PVM, PVS, PVX and PVY), reagents are also available for 5000 assays.