Sensitive and highly specific cDNA synthesis and qPCR in a single tube.
Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip® RNase inhibitor, and nuclease-free water.
* cDNA synthesis up to 60°C for superior specificity
* Analyze 1-4 targets in 1 reaction
* Reaction set-up and shipment without dry ice
* Robust amplification of GC-rich targets
* Contains dUTP to prevent cross-contamination when used in combination with UNG
* RNase inhibitor included in kit
SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.
Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don’t lose in sensitivity compared to singleplex or duplex assays.
