Highlights

Set of four organ-specific purified mouse genomic DNA standards from brain, kidney, liver, and thymus.
Precisely quantified levels of 5-mC or 5-hmC verified via mass spectrometry (LC/MS).
Useful control for detection methods of 5-mC or 5-hmC.

Description

The Mouse 5-hmC & 5-mC DNA Set is a set of organ specific mouse genomic DNAs isolated from a pool of 8-10 week old Swiss Webster mice. This genomic DNA set is an ideal control for detection and/or quantification methods against 5-methylcytosine and 5-hydroxymethylcytosine as both modified cytosines are present at physiologically relevant levels and loci. The levels of 5-mC and 5-hmC within the Mouse 5-hmC & 5-mC DNA Set have been precisely quantified by mass spectrometry (LC/MS) and been validated to be consistent by ELISA.

Technical Specifications

Concentration	250 ng/µl in modified TE buffer (10mM Tris-HCl; 0.1mM EDTA; pH 8.0)
Sample Source	Swiss Webster mice, 8-10 weeks old; Brain, Kidney, Liver, and Thymus
Sample Storage	≤ -20°C

Citations

Mouse 5-hmC & 5-mC DNA Set

D5019-1 / D5019-2 / D5019-3 / D5019-4 / D5019

Cat #	Name	Concentration
D5019-1	Mouse Brain DNA	5 µg
D5019-2	Mouse Kidney DNA	5 µg
D5019-3	Mouse Liver DNA	5 µg
D5019-4	Mouse Thymus DNA	5 µg
D5019	Mouse 5hmC & 5mC DNA Set	5 µg/20 µl

Documents

Protocol:

SDS (MSDS):