- Robust enrichment & immunoprecipitation of DNA containing 5mC.
- Includes a highly specific anti-5-methylcytosine monoclonal antibody for defined, reproducible results.
- Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses (e.g., assembling genomic libraries and determining genome-wide methylation status).
The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
|Applicable For||Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc.|
|Elution Volume||≥ 15 µl|
|Input||50 – 500 ng of DNA|
|Processing Time||4 hours|
Methylated-DNA IP Kit