Brief: Sulfonamides: The sulfa-related group of antibiotics, which are used to treat bacterial infection and some fungal infections. The sulfonamide family includes sulfadiazine, sulfamethizole (brand name: Thiosulfil Forte), sulfamethoxazole (Gantanol), sulfasalazine (Azulfidine), sulfisoxazole (Gantrisin), and various high-strength combinations of three sulfonamides. Sulfa drugs can have a number of potentially dangerous interactions with prescription and over-the-counter drugs (including PABA sunscreens), and are not appropriate for patients with some health conditions. Be sure your doctor knows about any other medications you take and your full health history before taking sulfonamides.

Principle: The Sulfonamides residues (SAs) ELISA test kit is based on the competitive enzyme immunoassay for the detection of Sulfonamides in the chicken, pork, milk, honey and egg, etc. The coupling antigens are pre-coated on the micro-well stripes. The Sulfonamides in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfonamides antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfonamides in the sample. This value is compared to the standard curve and the Sulfonamides concentration is subsequently obtained.

Sensitivity: 1 ppb
Detection limit: Tissue 1 ppb; Serum, urine 4 ppb; Milk 20 ppb
Recovery rate: Tissue 70 ±10%; Egg 65 ±10%; Milk, honey, serum 70 ±10%
Cross-reaction rate: Sulfadiazine (SD or SDZ) 100%; Sulfamonomethoxine (SMM) 97.6%; Sulfamerazine (SM1) 12%; Sulfamethoxazole (SMZ) 100%; Sulfathiazole (ST) 195.0%; Phthalylsulfathiazole (PST) 73%; Sulfamethyltiadiazolum 110%; Sulfapyridine 48%; Sulfamethoxydiazine (SMD) 26.4%; Sulfafurazole (SIZ) 95.0%