Potato Virus M (PVM) – Immunocapture RT-PCR Kits

Potato Virus M (PVM) – Immunocapture RT-PCR Kits


Potato Virus M (PVM) – Immunocapture RT-PCR Kits





PRODUCT DETAILS





Features of Immunocapture RT-PCR Kits:

  • ○ Pre-coated PCR tubes for capturing virus particles which can be directly amplified by RT-PCR, and allowing entire assay to be performed in a single PCR tube.
  • ○ Saving time by eliminating the time-consuming sample RNA extraction procedure, and saving fund by not using expensive RNA extraction kit.
  • ○ Obtaining robust and consistent result every time running a RT-PCR by virtually getting rid of all PCR inhibitors through viral immunocapturing process.
  • ○ Cost-effective and user-friendly for RT-PCR tests of large number of samples.

Brief Assay Procedure:

  • ○ Grind sample in PCR sample extraction buffer

  • ○ Dispense the ground sample into the tube of 1x8 PCR strip

  • ○ Prepare and add positive/negative controls into the control tube

  • ○ Incubate the plate for 2 hours at room temperature, or overnight at refrigerator

  • ○ Wash the PCR plate with PCR washing buffer

  • ○ Prepare and add the primers to master mix

  • ○ Add the master mix with enzymes into all of the PCR tubes

  • ○ Perform RT-PCR cycling






ORDERING INFORMATION






Catalog NumberDescriptionTest WellsShip Weight (Estimated)
V101-M1 ACDcap Immunocapture Kit for RT-PCR 48 1 lb (0.5 kg)
V101-M2 ACDcap Immunocapture Kit for RT-PCR 96 2 lb (1.0 kg)
V101-M3 ACDcap Immunocapture Kit for RT-PCR 288 3 lb (1.0 kg)
V101-M4 ACDamp Immunocapture RT-PCR Kit 48 1 lb (0.5 kg)
V101-M5 ACDamp Immunocapture RT-PCR Kit 96 2 lb (1.0 kg)
V101-M6 ACDamp Immunocapture RT-PCR Kit 288 3 lb (1.5 kg)
V101-P3 Positive Control 20 1 lb (0.5 kg)
V101-N3 Negative Control 20 1 lb (0.5 kg)
M029-1 Sample bag with PCR sample extraction buffer 50 1 lb (0.5 kg)
M029-2 Sample bag with PCR sample extraction buffer 100 2 lb (1.0 kg)
V101-M7 ACDcom Complete Immunocapture RT-PCR Kit 48 1 lb (0.5 kg)
V101-M8 ACDcom Complete Immunocapture RT-PCR Kit 96 2 lb (1.0 kg)
V101-M9 ACDcom Complete Immunocapture RT-PCR Kit 288 3 lb (1.5 kg)






Kit Components:






ACDcap Immunocapture Kit includes 1X8 PCR strips pre-coated with specific antibody, PCR sample extraction buffer, PCR plate washing buffer, positive control, negative control, and instruction.

ACDamp Immunocapture RT-PCR Kit includes 1X8 PCR strips pre-coated with specific antibody, PCR sample extraction buffer, PCR plate washing buffer, positive control, negative control, RT-PCR primers, and instruction.

ACDcom Complete Immunocapture RT-PCR Kit includes 1X8 PCR strips pre-coated with specific antibody, PCR sample extraction buffer, PCR plate washing buffer, positive control, negative control, RT-PCR primers, Master Mix, Reverse Transcriptase, Taq Polymerase, and instruction.




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