PRODUCT NAME

Phytophthora

SOURCE ANTIBODY

A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.

TEST FORMAT

TAS ELISA

REACTIVITY

This ELISA detects at least the following 16 Phytophthora species: P. cactorum, P. cambivora, P. capsici, P. cinnamomi, P. citricola, P. citrophthora, P. cryptogea, P. drechsleri, P. erythroseptica, P. fragariae, P. infestans, P. megasperma, P. palmivora, P. parasitica, and P. sojae. It reacted with all 39 isolates of Phytophthora infestans tested.
Reaction of this test is moderately strong. Optical Density at 405nm is in a range of 0.480 - 1.800 depending on the fungal titers in the samples tested.

SENSITIVITY

Sensitivity of the ELISA is moderately high. The fungus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.

SPECIFICITY

This test system reacts negatively with the following species of fungi: Pythium aphanidermatum, P. catenulatum, P. heterothalicum, P. irregulare, P. oligandrum, P. spinosum, P. splendens, P. torulosum, P. ultimum, P. ultimum var. ultimum. The test did not react with isolates of Aspergillussp., Fusarium sp., Monilinia sp., Penicilliumsp., Rhizoctonia sp., and Sclerotiniasp. There are moderate cross-reaction occurring with pure culture extracts of Pythium aristosporum, P. arrhenomanes, P. graminicola, P. paroecandrum, and P. violae, and weak cross-reaction occurring with pure culture extracts of Pythium multisporum, P. myriotylum, P. sylvaticum, P. tardicrescens, P. vanterpoolii.
There is no cross reaction with healthy plant tissues. Background is low on all of the negative control wells.

NAME

Phytophthora

ACRONYM

Phyt

SYNONYMS

GROUP/GENUS

Phytophthora

INFORMATION ABOUT THE VIRUS

Transmission: Air-born sporangia, infected plant tissues, seed, tuber, soil and agricultural activities.

Main host plants: Wide range of host plants including many important crops.
Fungal Structures: Sporangia, Sporangial spores, Mycelia.
Fungal Infection: The fungi infect leaves, stems, roots, tubers and other organs of host plants. The infected plant shows various symptoms including leaf lesions, stem cankers, root and tuber rotting, or the death of whole plant.