PHEA( Phenylethanolamine A) ELISA Kit

PHEA( Phenylethanolamine A) ELISA Kit

Manual (PDF)   |   MSDS (PDF)

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Catalog# E-FS-E015


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Product Details

Test Principle

This kit uses Competitive-ELISA as the method. It can detect Phenylethanolamine A (PHE A) in samples, such as muscle, feed. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, PHE A in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-PHE A antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of PHE A. The concentration of PHE A in the samples can be calculated by comparing the OD of the samples to the standard curve.

Product Specifications



Sample type

muscle, urine, feed




0.1 ppb (ng/mL)

Reaction mode

25℃, 30 min~30 min~15min

Detection limit

Urine ---0.1 ppb, Muscle ---0.1 ppb, Feed ---1 ppb

Cross reactivity

PHEA ---100%, Clenbuterol ---<1%, Salbutamol ---<1%, Ractopamine ---<1%

Sample recovery rate

Urine ---95%±15%, Muscle, Feed---85%±15%,

Incubation Temperature


Incubation Time


Kit Contains

  • ELISA Microtiter plate: 96 wells
  • Standard Liquid : 1 mL each
    (0ppb, 0.1ppb, 0.3ppb, 0.9ppb, 2.7ppb, 8.1ppb)
  • HRP Conjugate : 5.5 mL
  • Antibody Working Solution : 5.5 mL
  • Substrate Reagent A : 6 mL
  • Substrate Reagent B : 6 mL
  • Stop Solution : 6 mL
  • 20×Concentrated Wash Buffer : 40 mL
  • 2×Reconstitution Buffer : 50 mL
  • Plate Sealer : 3 pieces
  • Sealed Bag : 1 piece
  • Manual : 1 copy


Store the kit at 2~8℃. Do not freeze any test kit components.

Expiry date

The majority of ELISA kits have a shelf-life of 12 months from the production date.

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Other materials required but not supplied

Instruments: Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

Micropipette: Single channel (20-200 μL, 100-1000 μL), Multichannel (30-300 μL).

Reagents:NaOH, Ethyl acetate, Concentrated HCl, Acetonitrile, Methanol, N-hexane, Na2SO4.


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