PRODUCT NAME

Phakopsora

SOURCE ANTIBODY

A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.

TEST FORMAT

TAS ELISA

REACTIVITY

This ELISA reacts with P. pachyrhizi and P. meibomiae fungal species. The assay detected the isolates of P. pachyrhizi and P. meibomiae ininfected host plants such as soybean and lima bean. Reaction of this test is very strong. Optical Density at 405nm is in a range of 2.000 - >3.000 depending on the fungal titers in the samples tested.

SENSITIVITY

This ELISA is very sensitive, and can detect ASR/SR fungi in pre‐symptomatic plant leaves. The ASR/SR pathogen can be detected at dilution of 1:21870 for the plant leaf samples prepared at 14 days after infection, and at dilution of 1:270 for the plant leaf samples prepared at 5 days after infection.

SPECIFICITY

This test system is genius‐specific for Phakopsora, and reacts with both species of P. pachyrhizi (ASR) and P.meibomiae (SR). The antibodies used in this ELISA have high specificity to both r369 and r107 proteins.
This assaydoes not react with the other related rust fungi or any of the soybeanpathogens tested, including Uromyces appendisulatus, Puccinia punctiformis, Puccinia graminis, Puccinia graminis tritici, Rose rust, Fusarium graminearum, Downy mildew, Pseudomonas syringae , Pseudomonas syringae pv. phaseolicola, Xanthomonas campestris pv. glycine, Ustilago tritici, Erysiphae polygono DC, Septoria lycopersici and Cercospora sojina. There is no cross reaction with healthy plant tissues. Background is very low on all of the negative control wells.

NAME

Phakopsora

ACRONYM

Phak

SYNONYMS

GROUP/GENUS

INFORMATION ABOUT THE VIRUS