Onion Yellow Dwarf Virus (OYDV) – DAS ELISA

Onion Yellow Dwarf Virus (OYDV) – DAS ELISA


Onion Yellow Dwarf Virus (OYDV) – DAS ELISA

  • Test Format: DAS ELISA                                   
  • Group/Genus: Potyviruses

  • Datasheet      Tech Support


Catalog: V033
Name: Onion Yellow Dwarf Virus
Acronym: OYDV


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PRODUCT DETAILS






SPECIFICATIONS

PRODUCT NAME

Onion Yellow Dwarf Virus

SOURCE ANTIBODY

Rabbit polyclonal antibody for both capture and detection.

TEST FORMAT

DAS ELISA                                   

REACTIVITY

This test system reacted with all of the isolates of OYDV tested.  Reaction of the ELISA is very strong.  Optical Density at 405nm is in a range of 2.200 - >3.000 depending on the virus titer in the samples tested.

SENSITIVITY

Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted at 1:2430.

SPECIFICITY

This test shows no cross-reaction with other potyviruses such as DsMV, LMV, LYSV, MDMV, SCMV, PepMoV, PPV, PRSV, PVV, SbMV, SYSV, TEV, TuMV, TVMV, WMV2, and ZYMV. There is no cross reaction found with healthy plant tissues.  Background is low on all of the negative control wells.


INFORMATION ABOUT THE VIRUS

NAME

Onion Yellow Dwarf Virus

ACRONYM

OYDV

SYNONYMS

Allium virus 1, Marmor cepae.

GROUP/GENUS

Potyviruses

INFORMATION ABOUT THE VIRUS


Vector:  Aphids, including Green Peach aphid, Myzus persicae, and other aphid species.
Main host plants:  Onion, garlic leek and shallot.Virus Particle:  Filamentous particles containing RNA genome
Virus Infection:  Onion Yellow Dwarf Virus (OYDV) infects onion (Allium cepa), shallot (Allium cepa var. ascalonicum), garlic (Allium sativum), and leek (Allium porrum), and causes yield losses in these Allium crops.  Common symptoms of infected onion include leaf striping and plant stunting.  OYDV induces leaf mosaic in garlic and is part of the garlic mosaic virus complex.






ORDERING INFORMATION






Catalog NumberDescriptionTest WellsShip Weight (Estimated)
V033-R1ELISA Reagents, Alkaline Phosphatase5002 lb (1 kg)
V033-R2ELISA Reagents, Alkaline Phosphatase10003 lb (1.5 kg)
V033-R3ELISA Reagents, Alkaline Phosphatase500012 lb (6 kg)
V033-R4ELISA Reagents, Alkaline Phosphatase1001 lb (0.5 kg)
V033-K1ELISA Kit, Alkaline Phosphatase5002 lb (1 kg)
V033-K2ELISA Kit, Alkaline Phosphatase10003 lb (1.5 kg)
V033-C1Coating Antibody5002 lb (1 kg)
V033-C2Coating Antibody10002 lb (1 kg)
V033-C3Coating Antibody50002 lb (1 kg)
V033-D1Detecting conjugate, Alkaline Phosphatase5002 lb (1 kg)
V033-D2Detecting conjugate, Alkaline Phosphatase10002 lb (1 kg)
V033-D3Detecting conjugate, Alkaline Phosphatase50002 lb (1 kg)
V033-P1Positive control181 lb (0.5 kg)
V033-N1Negative control181 lb (0.5 kg)
M004-1Buffers for DAS/TAS, alkaline phosphatase5002 lb (1.0 kg)
M004-2Buffers for DAS/TAS, alkaline phosphatase10003 lb (1.5 kg)
M004-3Buffers for DAS/TAS, alkaline phosphatase50006 lb (3.0 kg)






REFERENCES






1.  CMI/AAB Descriptions of Plant Viruses, No. 158.
2.  Koch, M. and Saloman, R. 1994. Serological detection of onion yellow dwarf virus in garlic. Plant Dis. 78:785-788.
3.  Lot, H., Chovelon, V., S., and Delecolle, B. 1998. Effects of onion yellow dwarf and leek yellow stripe viruses on symptomology and yield loss of three French garlic cultivars. Plant Dis. 82:1381-1385.

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