Okra Mosaic Virus (OkMV) – DAS ELISA   

Okra Mosaic Virus (OkMV) – DAS ELISA   


Okra Mosaic Virus (OkMV) – DAS ELISA   

  • Test Format: DAS ELISA                                   
  • Group/Genus: Tymovirus

  • Datasheet      Tech Support


Catalog: V161
Name: Okra Mosaic Virus
Acronym: OkMV


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PRODUCT DETAILS






SPECIFICATIONS

PRODUCT NAME

Okra Mosaic Virus

SOURCE ANTIBODY

Rabbit polyclonal antibody for both capture and detection

TEST FORMAT

DAS ELISA                                   

REACTIVITY

 The test reacted with all OkMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.750 - >2.700 depending on the virus titer in the samples tested.

SENSITIVITY

Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

SPECIFICITY

There is no cross reaction with healthy plant  tissues such as okra or other host plants. Background is low on all of  the negative control wells.


INFORMATION ABOUT THE VIRUS

NAME

Okra Mosaic Virus

ACRONYM

OkMV

SYNONYMS

 virus de la mosaique du Gombo

GROUP/GENUS

Tymovirus

INFORMATION ABOUT THE VIRUS


Vector:  Transmitted by a vector; an insect; Podagrica decolorata in C“te d'Ivoire (Givord and den Boer, 1980); Podagrica uniforma, P. sjostedti in Nigeria (Lana and Taylor, 1976; Atiri, 1984). Possibly Bemisia tabaci is a vector in Nigeria (Lana and Taylor, 1976), but needs confirmation; Coleoptera. Not transmitted by Aphis gossypii, Chrysolagria cuprina, Lagria villosa, Medythia quaterna, Ootheca mutabilis, Nisotra dilecta. Transmitted in a non-persistent manner.
Main natural host plants: Abelmoschus esculentus, Hibiscus rosa-sinensis, Abutilon hirtum, Borreria intricans, Corchorus olitorius, Hibiscus sabdariffa, Malvastrum coromandelianum, Sida acuta, S. rhomboidea, Physalis angulata, Sida linifolia - mosaic and vein banding, Urena lobata.Virus Infection:  Symptoms persist (in all other families except Malvaceae). Symptoms mosaic, vein chlorosis and banding in Malvaceae. Other families show interveinal chlorosis, vein chlorosis and vein banding. In all families plants are often stunted.
Diagnostically susceptible host species and symptoms: Chenopodium amaranticolor - systemic chlorotic spotting and line patterns; Cucumis sativus - large chlorotic local lesions, then systemic vein chlorosis; Arachis hypogaea - dark and light green mosaic; Vigna unguiculata - fine systemic vein chlorosis and mosaic; Nicotiana clevelandii - systemic dark green and whitish mosaic, dwarfing.
Geographical distribution: Spreads in Cote d'Ivoire and Nigeria.






ORDERING INFORMATION






Catalog Number Description Test Wells Ship Weight (Estimated)
V161-R1 ELISA Reagents, Alkaline Phosphatase 500 2 lb (1 kg)
V161-R2 ELISA Reagents, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V161-R3 ELISA Reagents, Alkaline Phosphatase 5000 12 lb (6 kg)
V161-R4 ELISA Reagents, Alkaline Phosphatase 100 1 lb (0.5 kg)
V161-K1 ELISA Kit, Alkaline Phosphatase 500 2 lb (1 kg)
V161-K2 ELISA Kit, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V161-C1 Coating Antibody 500 2 lb (1 kg)
V161-C2 Coating Antibody 1000 2 lb (1 kg)
V161-C3 Coating Antibody 5000 2 lb (1 kg)
V161-D1 Detecting conjugate, Alkaline Phosphatase 500 2 lb (1 kg)
V161-D2 Detecting conjugate, Alkaline Phosphatase 1000 2 lb (1 kg)
V161-D3 Detecting conjugate, Alkaline Phosphatase 5000 2 lb (1 kg)
V161-P2 ELISA Control, Positive 18 1 lb (0.5 kg)
V161-N1 Negative control 18 1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 6 lb (3.0 kg)






REFERENCES






1.  Atiri, G.I., Ivbijaro, M.F. and Oladele, A.D. (1991). Trop. Agric., Trin. 68: 178.
2.  Givord, L. and Koenig, R. (1974). CMI/AAB Descr. Pl. Viruses No. 128, 4 pp.

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