Brief: Furacili is synthetic broad-spectrum antibiotic and can treat animal desease widely , which is frequently employed in animal and aquaculture production. But Furacilin and its metabolite residues in animal food are passed to human through the food chain , taking them regularly cause various diseases, which can lead to cancer, teratogenesis and other side effects. Furacilin was banned from use in food industry in Australia, Canada, Japan, Singapore, and analsysis of Nitrofuran was strictly implemented in Aquatic Products. Japanese positive list regulated that Nitrofuran medicine mustn’t be detected out. The use of Nitrofurantoin was are classified as forbidden drugs in Veterinary medicine And Other Compound Detailed Banned in Food Animal Lists which was pulished by Ministry of Chinese Agriculture in March 2002.
SEM-residues are determined most commonly by HPLC, LC-MS before. Enzyme immunoassays, compared with chromatographic methods, show quite advantages regarding sensitivty , simple process , technical equipment and time requirement.

Principle: The Semicarbazide (SEM) ELISA test Kit is based on the competitive enzyme immunoassay for the detection of Semicarbazide (SEM) in the tissue(chicken, fish, shrimp), milk, honey, whole egg and so on. The coupling antigen is pre-coated on the micro-well stripes. The SEM in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against SEM. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample is inversely proportional to the SEM in the sample. This value is compared to the standard curve and the content of the corresponding SEM is subsequently obtained.

Sensitivity: 0.1 ppb
Detection limit: Tissue, honey, egg 0.2 ppb; Fish, shrimp (some interference in shrimp and fish) 0.3 ppb
Recovery rate: Shrimp and fish 85±10%; Honey, meat, liver, egg 75±15%
Cross-reaction rate: SEM 100%; AHD ﹤0.1%; AMOZ ﹤0.1%; AOZ ﹤0.1%