Brief: Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin, and related compounds. The Food and Drug Administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. The rule went into effect on May 7, 2002 and is the result of evidence that the drugs may induce carcinogenic residues in animal tissues.

Principle: The Furaltadone (AMOZ) ELISA test kit is based on the competitive enzyme immunoassay for the detection of AMOZ (3-anmino-5-morpholinomethyl) in the tissue (chicken, fish, shrimp and so on). The coupling antigen is pre-coated on the micro-well stripes. The AMOZ in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against AMOZ. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of AMOZ in it. This value is compared to the standard curve and the content of the corresponding AMOZ is subsequently obtained.

Sensitivity: 0.1 ppb
Detection limit: Fish/shrimp (some interference in shrimp and fish) 0.3 ppb; Tissue, honey 0.2 ppb
Recovery rate: Shrimp and fish90±15%; Honey, chicken/liver80±15%
Cross-reaction rate: AMOZ100%; AHD﹤0.1%; AOZ﹤0.1%; SEM﹤0.1%