Brief: Nitrofurans are a class of drugs typically used as antibiotics or antimicrobials. The defining structural component is a furan ring with a nitro group.
Members of this class of drugs include:
1. Furazolidone, antibacterial
2. Furylfuramide
3. Nitrofurantoin, antibacterial
4. Nitrofurazone, antibacterial
5. Nifuratel
6. Nifurtoinol, antibacterial
7. Nifuroxazide, antibiotic
8. Nifurtimox, antiparasitic
9. Nifurzide, anti-infective

Principle: The Nitrofurantoin (AHD) ELISA test kit is based on the competitive enzyme immunoassay for the detection of Aminohydantoin (AHD) in the tissue (chicken, meat, fish and shrimp). The coupling antigen is pre-coated on the micro-well stripes. The Aminohydantoin (AHD) in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against AHD. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of AHD in it. This value is compared to the standard curve and the content of the corresponding AHD is subsequently obtained.

Sensitivity: 0.05 ppb
Detection limit: Tissue and honey 0.1 ppb; Shrimp and fish (some interference in shrimp and fish) 0.15 ppb
Recovery rate: Shrimp and fish 90±15%; Honey, meat, liver 80±15%
Cross-reaction rate: AHD 100%; AMOZ ﹤0.1%; AOZ ﹤0.1%; SEM ﹤0.1%