Brief: Neomycin, an antibiotic, is used to prevent or treat skin infections caused by bacteria. It is not effective against fungal or viral infections. Neomycin may cause damage to the kidneys and/or nerves. It’s potentially toxic compounds causing significant damage in vestibular andauditory functions in humans as well as in animals.

Principle: The Neomycin ELISA test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained.

Sensitivity: 0.5 ppb
Detection limit: Honey 10 ppb; Chicken/liver, pork/liver, fish, shrimp 10 ppb
Recovery rate: Chicken, pork, honey, fish, shrimp 85±15%; Liver (chicken or porcine) 70±10%
Cross-reaction rate: Neomycin 100%; Streptomycin < 0.1%; Gentamicin < 0.1%; Dihydrostreptomycin < 0.1%; Kanamycin < 0.1%; Tobramycin < 0.1%; Sisomicin < 0.1%