Mirafiori Lettuce Big Vein Virus (MiLBVV) – DAS ELISA

Mirafiori Lettuce Big Vein Virus (MiLBVV) – DAS ELISA


Mirafiori Lettuce Big Vein Virus (MiLBVV) – DAS ELISA



Catalog: V275
Name: Mirafiori Lettuce Big Vein Virus
Acronym: MiLBVV


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PRODUCT DETAILS






SPECIFICATIONS

PRODUCT NAME

Mirafiori Lettuce Big Vein Virus

SOURCE ANTIBODY

Rabbit polyclonal antibody for both capture and detection.

TEST FORMAT

   DAS ELISA

REACTIVITY

 Reaction of the ELISA is strong.  Optical Density at 405nm is in a range of 0.900 – 2.400 depending on the virus titer in the samples tested.

SENSITIVITY

Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 2430.

SPECIFICITY

This test system has no cross reaction with healthy plant tissues tested. Background is low on the negative control wells.


INFORMATION ABOUT THE VIRUS

NAME

Mirafiori Lettuce Big Vein Virus

ACRONYM

MiLBVV

SYNONYMS

GROUP/GENUS

INFORMATION ABOUT THE VIRUS

Main Diseases: Symptoms vary seasonally. Symptoms vein yellowing then chlorotic or white vein banding. Infection of Lactuca sativa show chlorotic or white vein banding.
Transmission: Transmitted by a vector; a fungus; Olpidium brassicae; Chytridiales. Not transmitted by Myzus persicae, Hyperomyzus lactuca, Xiphinema spp., Longidorus spp. Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.Geographical Distribution: Spreads in Australia, the former Czechoslovakia, Italy, Japan, the Netherlands, New Zealand, the UK, and the USA.






ORDERING INFORMATION






Catalog Number Description Test Wells Ship Weight (Estimated)
V275-R1 ELISA Reagents, Alkaline Phosphatase 500 2 lb (1 kg)
V275-R2 ELISA Reagents, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V275-R3 ELISA Reagents, Alkaline Phosphatase 5000 12 lb (6 kg)
V275-R4 ELISA Reagents, Alkaline Phosphatase 100 1 lb (0.5 kg)
V275-K1 ELISA Kit, Alkaline Phosphatase 500 2 lb (1 kg)
V275-K2 ELISA Kit, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V275-C1 Coating Antibody 500 2 lb (1 kg)
V275-C2 Coating Antibody 1000 2 lb (1 kg)
V275-C3 Coating Antibody 5000 2 lb (1 kg)
V275-D1 Detecting conjugate, Alkaline Phosphatase 500 2 lb (1 kg)
V275-D2 Detecting conjugate, Alkaline Phosphatase 1000 2 lb (1 kg)
V275-D3 Detecting conjugate, Alkaline Phosphatase 5000 2 lb (1 kg)
V275-P1 Positive control 18 1 lb (0.5 kg)
V275-N1 Negative control 18 1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 6 lb (3.0 kg)






REFERENCES






1. Allen, M.W. (1948). Phytopathology 38: 616.
2. Campbell, R.N. (1985). Can. J. Bot. 63: 2288.
3. Tomlinson, J.A. and Garrett, R.G. (1964). Ann. appl. Biol. 54: 45.
4. Umar M, Amer MA, Al-Saleh MA, Al-Shahwan IM, Shakeel MT, Zakri AM, Katis NI. Arch Virol. 2017 Jul;162(7):2067-2072.
5. Vetten, H.J., Lesemann, D.-E. and Dalchow, J. (1987). J. Phytopath. 120: 53.

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