Melon Necrotic Spot Virus (MNSV) – DAS ELISA

Melon Necrotic Spot Virus (MNSV) – DAS ELISA


Melon Necrotic Spot Virus (MNSV) – DAS ELISA

  • Test Format: DAS ELISA                                   
  • Group/Genus: Carmoviruses

  • Datasheet      Tech Support


Catalog: V031
Name: Melon Necrotic Spot Virus
Acronym: MNSV


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PRODUCT DETAILS






SPECIFICATIONS

PRODUCT NAME

Melon Necrotic Spot Virus

SOURCE ANTIBODY

Rabbit polyclonal antibody for both capture and detection

TEST FORMAT

DAS ELISA                                   

REACTIVITY

This test system reacted with all of the isolates or strains of MNSV tested.  Reaction of the ELISA is very strong.  Optical Density at 405nm is in a range of 1.200 - 2.800 depending on the virus titer in the samples tested.

SENSITIVITY

Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:810.

SPECIFICITY

This ELISA shows no cross-reaction with other carmoviruses, such as carnation mottle virus, hibiscus chlorotic ringspot virus and pelargonium flower break virus. There is no cross reaction found with healthy cucurbit plant tissues.  Background is low on all of the negative control wells.


INFORMATION ABOUT THE VIRUS

NAME

Melon Necrotic Spot Virus

ACRONYM

MNSV

SYNONYMS

GROUP/GENUS

Carmoviruses

INFORMATION ABOUT THE VIRUS


Vector:  Fungus
Main host plants:  CucurbitsVirus Particle:  Isometric particles containing RNA genome, 30 nm in diameter. 
Virus Infection:  Melon necrotic spot virus (MNSV) occurs in Japan, the Netherlands, the UK, and the USA.  MNSV is transmitted by the chytrid fungus Olpidium radicaleand in soil.  This virus is also transmitted by mechanical sap inoculation.  Naturally, MNSV occurs only in greenhouse melons and cucumbers, and causes severe yield loss.  The symptoms induced by the virus include necrotic spots or large necrotic lesions on the leaves, and necrosis on the stems of greenhouse melons and cucumbers.






ORDERING INFORMATION






Catalog Number Description Test Wells Ship Weight (Estimated)
V031-R1 ELISA Reagents, Alkaline Phosphatase 500 2 lb (1 kg)
V031-R2 ELISA Reagents, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V031-R3 ELISA Reagents, Alkaline Phosphatase 5000 12 lb (6 kg)
V031-R4 ELISA Reagents, Alkaline Phosphatase 100 1 lb (0.5 kg)
V031-K1 ELISA Kit, Alkaline Phosphatase 500 2 lb (1 kg)
V031-K2 ELISA Kit, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V031-C1 Coating Antibody 500 2 lb (1 kg)
V031-C2 Coating Antibody 1000 2 lb (1 kg)
V031-C3 Coating Antibody 5000 2 lb (1 kg)
V031-D1 Detecting conjugate, Alkaline Phosphatase 500 2 lb (1 kg)
V031-D2 Detecting conjugate, Alkaline Phosphatase 1000 2 lb (1 kg)
V031-D3 Detecting conjugate, Alkaline Phosphatase 5000 2 lb (1 kg)
V031-P1 Positive control 18 1 lb (0.5 kg)
V031-N1 Negative control 18 1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 6 lb (3.0 kg)






REFERENCES






1.  CMI/AAB Descr. Pl. Viruses, No. 302, 1985.
2.  Furuki, Tech. Bull. Schizuoka Agric. Exp. Stn 14:94, 1981.
3.  Gonzalez-Garza, Gumpf, Kishaba and Bohn, Phytopathology 69:340, 1979.

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