Garlic Common Latent Virus (GCLV) – Immunocapture RT-PCR Kits

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Catalog: V026

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PRODUCT DETAILS

Features of Immunocapture RT-PCR Kits:

○ Pre-coated PCR tubes for capturing virus particles which can be directly amplified by RT-PCR, and allowing entire assay to be performed in a single PCR tube.
○ Saving time by eliminating the time-consuming sample RNA extraction procedure, and saving fund by not using expensive RNA extraction kit.
○ Obtaining robust and consistent result every time running a RT-PCR by virtually getting rid of all PCR inhibitors through viral immunocapturing process.
○ Cost-effective and user-friendly for RT-PCR tests of large number of samples.

Brief Assay Procedure:
○ Grind sample in PCR sample extraction buffer ○ Dispense the ground sample into the tube of 1x8 PCR strip ○ Prepare and add positive/negative controls into the control tube ○ Incubate the plate for 2 hours at room temperature, or overnight at refrigerator ○ Wash the PCR plate with PCR washing buffer ○ Prepare and add the primers to master mix ○ Add the master mix with enzymes into all of the PCR tubes ○ Perform RT-PCR cycling

Brief Assay Procedure:

○ Grind sample in PCR sample extraction buffer

○ Dispense the ground sample into the tube of 1x8 PCR strip

○ Prepare and add positive/negative controls into the control tube

○ Incubate the plate for 2 hours at room temperature, or overnight at refrigerator

○ Wash the PCR plate with PCR washing buffer

○ Prepare and add the primers to master mix

○ Add the master mix with enzymes into all of the PCR tubes

○ Perform RT-PCR cycling

ORDERING INFORMATION

Catalog Number	Description	Test Wells	Ship Weight (Estimated)
V026-M1	ACDcap Immunocapture Kit for RT-PCR	48	1 lb (0.5 kg)
V026-M2	ACDcap Immunocapture Kit for RT-PCR	96	2 lb (1.0 kg)
V026-M3	ACDcap Immunocapture Kit for RT-PCR	288	3 lb (1.0 kg)
V026-M4	ACDamp Immunocapture RT-PCR Kit	48	1 lb (0.5 kg)
V026-M5	ACDamp Immunocapture RT-PCR Kit	96	2 lb (1.0 kg)
V026-M6	ACDamp Immunocapture RT-PCR Kit	288	3 lb (1.5 kg)
V026-P3	Positive Control	20	1 lb (0.5 kg)
V026-N3	Negative Control	20	1 lb (0.5 kg)
M029-1	Sample bag with PCR sample extraction buffer	50	1 lb (0.5 kg)
M029-2	Sample bag with PCR sample extraction buffer	100	2 lb (1.0 kg)

Kit Components:

ACDcap Immunocapture Kit includes 1X8 PCR strips pre-coated with specific antibody, PCR sample extraction buffer, PCR plate washing buffer, positive control, negative control, and instruction.

ACDamp Immunocapture RT-PCR Kit includes 1X8 PCR strips pre-coated with specific antibody, PCR sample extraction buffer, PCR plate washing buffer, positive control, negative control, RT-PCR primers, and instruction.

ACDcom Complete Immunocapture RT-PCR Kit includes 1X8 PCR strips pre-coated with specific antibody, PCR sample extraction buffer, PCR plate washing buffer, positive control, negative control, RT-PCR primers, Master Mix, Reverse Transcriptase, Taq Polymerase, and instruction.

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