Brief: Florfenicol is a broad spectrum antibiotic which is active against both Gram-positive and Gram-negative bacteria isolated from domestic animals. The GreenSpring™ Florfenicol (FF) ELISA Kit is the first enzyme immunoassay to test Florfenicol in food in the world.

Principle: The Florfenicol (FF) ELISA Kit is based on the competitive enzyme immunoassay for the detection of Florfenicol in the tissue (chicken, pork), fish, shrimp , honey. The coupling antigen is pre-coated on the micro-well stripes. The Florfenicol in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against Florfenicol. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Florfenicol in it. This value is compared to the standard curve and the content of the corresponding Florfenicol is subsequently obtained.

Sensitivity : 0.5 ppb
Detection limit: Honey 1.5 ppb; Meat, liver, shrimp, fish 0.5 ppb;
Recovery rate: Honey 70 ±10%; Meat, liver, shrimp, fish 80 ±10%
Cross-reaction rate: Florfenicol 100%; Florfenicol Amine 11%; Thiamphenicol < 0.1%; Chloramphenicol < 0.1%