Brief: Enrofloxacin is a broad spectrum bactericidal antibiotic. Although the mechanism of action is not well understood, Enrofloxacin is effective against a broad spectrum of gram positive and gram negative bacteria. Enrofloxacin is eliminated by both renal and hepatic metabolism. Animals with impaired kidney or liver function may need extra monitoring and dose adjustments to prevent excess drug accumulation.

Principle: The Enrofloxacin ELISA Test Kit is based on the competitive enzyme immunoassay. The enzyme conjugate is pre-coated on the micro-well stripes. The residue enrofloxacin in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against enrofloxacin. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of residue enrofloxacin in it. This value is compared to the standard curve and the content of the corresponding enrofloxacin residue is subsequently obtained.

Sensitivity: 0.5ppb
Detection limit: Tissues (chicken, chicken liver, pork, pork liver, shrimp and fish) 1ppb; Serum 1ppb; Honey 2ppb
Cross-reaction rate: Enrofloxacin 100%; Ciprofloxacin 1.25%; Norfloxacin 1.65%; Other Xacin medicines less than 0.1%
Recovery rate: Tissues (chicken, chicken liver, pork, pork liver, shrimp and fish) 85±15%; Serum 80±15%; Honey 75±15%