Components of ELISA Kit
| Coating Antibody | Specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) for coating ELISA plate. |
| Detecting Conjugate | Specific antibody enzyme conjugate, or anti-species antibody enzyme conjugate. |
| ELISA Plate | Empty ELISA plate with high binding capacity. The plate should be used for our ELISA Reagents to achieve a maximum test results. |
| Controls | Positive control and negative control |
| Buffers | Coating buffer, sample buffer, enzyme conjugate buffer, washing buffer, substrate buffer and PNP tablets. |
| Instruction | Specific test instructions for each of the three different ELISA Formats. |
Contents of ELISA Reagents
| Item | 500 wells | 1000 wells | Storage |
| Coating antibody | 0.25 ml | 0.50 ml | 4 oC |
| Detecting conjugate, alkaline phosphatase | 0.25 ml | 0.50 ml | 4 oC |
| 96-well ELISA plates | 5 | 10 | Room temperature |
| Positive control | 1 | 2 | 4 oC |
| Negative control | 1 | 2 | 4 oC |
| Coating buffer, 10x | 6 ml | 12 ml | Room temperature |
| Sample buffer powder | 11.6 g | 23.2 g | Room temperature |
| Enzyme conjugate buffer, 10x | 6 ml | 12 ml | Room temperature |
| PBST Washing buffer powder | 28.7 g | 57.23g | Room temperature |
| Tween-20 for sample/washing buffers | 7.0 g | 14.0 g | Room temperature |
| PNP substrate buffer, 5x | 10 ml | 20 ml | 4 oC |
| PNP tablet, 5mg each | 10 tablets | 20 tablets | -10-20 oC |
| Instruction | 1 | 1 |
| Item | 500 wells | 1000 wells | Storage |
| Coating antibody | 0.25 ml | 0.50 ml | 4 oC |
| Detecting conjugate, alkaline phosphatase | 0.25 ml | 0.50 ml | 4 oC |
| 96-well ELISA plates | 5 | 10 | Room temperature |
| Positive control | 1 | 2 | 4 oC |
| Negative control | 1 | 2 | 4 oC |
| Coating buffer, 10x | 6 ml | 12 ml | Room temperature |
| Sample buffer powder | 11.6 g | 23.2 g | Room temperature |
| Enzyme conjugate buffer, 10x | 6 ml | 12 ml | Room temperature |
| PBST Washing buffer powder | 28.7 g | 57.23g | Room temperature |
| Tween-20 for sample/washing buffers | 7.0 g | 14.0 g | Room temperature |
| PNP substrate buffer, 5x | 10 ml | 20 ml | 4 oC |
| PNP tablet, 5mg each | 10 tablets | 20 tablets | -10-20 oC |
| Instruction | 1 | 1 |
| Item | 500 wells | 1000 wells | Storage |
| Detecting conjugate, alkaline phosphatase, | 0.25 ml (A) | 0.50 ml (A) | 4 oC |
| (Bottles A and B) | |||
| 0.25 ml (B) | 0.50 ml (B) | ||
| 96-well ELISA plates | 5 | 10 | Room temperature |
| Positive control | 1 | 2 | 4 oC |
| Negative control | 1 | 2 | 4 oC |
| Sample buffer powder | 11.6 g | 23.2 g | Room temperature |
| Enzyme conjugate buffer, 10x | 6 ml | 12 ml | Room temperature |
| PBST Washing buffer powder | 28.7 g | 57.23g | Room temperature |
| Tween-20 for washing buffers | 7.0 g | 14.0 g | Room temperature |
| PNP substrate buffer, 5x | 10 ml | 20 ml | 4 oC |
| PNP tablet, 5mg each | 10 tablets | 20 tablets | -10-20 oC |
| Instruction | 1 | 1 |
