Asparagus Virus – 2 (AV-2) – DAS ELISA  

Asparagus Virus – 2 (AV-2) – DAS ELISA  


Asparagus Virus – 2 (AV-2) – DAS ELISA  

  • Test Format: DAS ELISA                                   
  • Group/Genus: Ilarvirus

  • Datasheet      Tech Support


Catalog: V205
Name: Asparagus Virus - 2
Acronym: AV-2


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PRODUCT DETAILS






SPECIFICATIONS

PRODUCT NAME

Asparagus Virus - 2

SOURCE ANTIBODY

Rabbit polyclonal antibody for both capture and detection.

TEST FORMAT

DAS ELISA                                   

REACTIVITY

 The test reacted with all AV-2 isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.860 - 2.530 depending  on the virus titer in the samples tested.

SENSITIVITY

Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

SPECIFICITY

There is no cross reaction with healthy plant  tissues such as asparagus and other host plants. Background is relative low on all of  the negative control wells.


INFORMATION ABOUT THE VIRUS

NAME

Asparagus Virus - 2

ACRONYM

AV-2

SYNONYMS

asparagus virus C, asparagus latent virus.

GROUP/GENUS

Ilarvirus

INFORMATION ABOUT THE VIRUS


Vector: Not transmitted by Myzus persicae.
Main natural host plants: Asparagus officinalisVirus Infection: Cause stunting, decline of plants. Symptoms persist
Diagnostically susceptible host species and symptoms: Chenopodium amaranticolor, C. quinoa - local necrotic spots, systemic necrosis; Gomphrena globosa - white spots with red borders; Nicotiana tabacum cv. Samsun - local necrotic rings and systemic vein yellowing and necrotic lines; Nicotiana tabacum cv. White Burley - local necrotic rings.
Geographical distribution: Spreads in the Eurasian region and the North American region. Found, but with no evidence of spread, in Europe and North America; Denmark, Germany, Italy, the UK and the USA.






ORDERING INFORMATION






Catalog Number Description Test Wells Ship Weight (Estimated)
V205-R1 ELISA Reagents, Alkaline Phosphatase 500 2 lb (1 kg)
V205-R2 ELISA Reagents, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V205-R3 ELISA Reagents, Alkaline Phosphatase 5000 12 lb (6 kg)
V205-R4 ELISA Reagents, Alkaline Phosphatase 100 1 lb (0.5 kg)
V205-K1 ELISA Kit, Alkaline Phosphatase 500 2 lb (1 kg)
V205-K2 ELISA Kit, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V205-C1 Coating Antibody 500 2 lb (1 kg)
V205-C2 Coating Antibody 1000 2 lb (1 kg)
V205-C3 Coating Antibody 5000 2 lb (1 kg)
V205-D1 Detecting conjugate, Alkaline Phosphatase 500 2 lb (1 kg)
V205-D2 Detecting conjugate, Alkaline Phosphatase 1000 2 lb (1 kg)
V205-D3 Detecting conjugate, Alkaline Phosphatase 5000 2 lb (1 kg)
V205-P2 ELISA Control, Positive 18 1 lb (0.5 kg)
V205-N1 Negative control 18 1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 6 lb (3.0 kg)






REFERENCES






1.  Bertaccini, A., Giunchedi, L. and Poggi Pollini, C. (1990). Acta Hort. 271: 279.
2.  Uyeda, I. and Mink, G.I. (1984). CMI/AAB Descr. Pl. Viruses No.288, 4 pp.

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