Apple Stem Grooving Virus (ASGV) – DAS ELISA 

Apple Stem Grooving Virus (ASGV) – DAS ELISA 


Apple Stem Grooving Virus (ASGV) – DAS ELISA 



Catalog: V203
Name: Apple Stem Grooving Virus
Acronym: ASGV


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PRODUCT DETAILS






SPECIFICATIONS

PRODUCT NAME

Apple Stem Grooving Virus

SOURCE ANTIBODY

A rabbit polyclonal antibody for both capture and detection.

TEST FORMAT

DAS ELISA             

REACTIVITY

 The test reacted with all ASGV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.400 - >1.700 depending on the virus titer in the samples tested.

SENSITIVITY

Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:30 -1:90.

SPECIFICITY

There is no cross reaction with healthy plant  tissues such as apple or other host plants. Background is low on all of  the negative control wells.


INFORMATION ABOUT THE VIRUS

NAME

Apple Stem Grooving Virus

ACRONYM

ASGV

SYNONYMS

 Virginia Crab stem grooving virus, chenopodium dark green epinasty virus, brown line disease virus.

GROUP/GENUS

Capillovirus

INFORMATION ABOUT THE VIRUS


Vector:  Transmitted by means not involving a vector.
Main natural host plants: Malus sylvestris Virus Infection Symptoms: Virginia Crab Apple- stem grooves, abnormal graft union.  Symptoms in Virginia Crab apple persist.
Diagnostically susceptible host species and symptoms: Malus sylvestris cv. Virginia Crab - grooves in the wood. Strain `E-36' causes graft union to be brown, fragile, swollen and sunken areas on the stem; Chenopodium quinoa - etched necrotic local lesions, systemic chlorotic rings and mottle, tip malformed; some isolates induce epinasty; Nicotiana glutinosa - systemic yellow mosaic, line patterns; Phaseolus vulgaris - purple brown spots, chlorotic local lesions, systemic necrosis.
Geographical distribution: Probably distributed worldwide. Spreads in Australia, China, India, Italy, Japan, the Netherlands, New Zealand, Portugal, and the UK (probably occurs wherever apple trees are cultivated).






ORDERING INFORMATION






Catalog NumberDescriptionTest WellsShip Weight (Estimated)
V203-R1ELISA Reagents, Alkaline Phosphatase5002 lb (1 kg)
V203-R2ELISA Reagents, Alkaline Phosphatase10003 lb (1.5 kg)
V203-R3ELISA Reagents, Alkaline Phosphatase500012 lb (6 kg)
V203-R4ELISA Reagents, Alkaline Phosphatase1001 lb (0.5 kg)
V203-K1ELISA Kit, Alkaline Phosphatase5002 lb (1 kg)
V203-K2ELISA Kit, Alkaline Phosphatase10003 lb (1.5 kg)
V203-C1Coating Antibody5002 lb (1 kg)
V203-C2Coating Antibody10002 lb (1 kg)
V203-C3Coating Antibody50002 lb (1 kg)
V203-D1Detecting conjugate, Alkaline Phosphatase5002 lb (1 kg)
V203-D2Detecting conjugate, Alkaline Phosphatase10002 lb (1 kg)
V203-D3Detecting conjugate, Alkaline Phosphatase50002 lb (1 kg)
V203-P1Positive control181 lb (0.5 kg)
V203-N1Negative control181 lb (0.5 kg)
M004-1Buffers for DAS/TAS, alkaline phosphatase5002 lb (1.0 kg)
M004-2Buffers for DAS/TAS, alkaline phosphatase10003 lb (1.5 kg)
M004-3Buffers for DAS/TAS, alkaline phosphatase50006 lb (3.0 kg)






REFERENCES






1.  de Sequeira, O.A. and Lister, R.M. (1969b). Phytopathology 59: 1740.
2.  Lister, R.M. (1970). CMI/AAB Descr. Pl. Viruses No. 31, 4 pp.
3.  Yoshikawa, N. Sasaki, E., Kato, M. and Takahashi, T. (1992). Virology 191: 98.

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