Apple Chlorotic Leaf Spot Virus (ACLSV) – DAS ELISA

Apple Chlorotic Leaf Spot Virus (ACLSV) – DAS ELISA

Apple Chlorotic Leaf Spot Virus (ACLSV) – DAS ELISA

  • Test Format: DAS ELISA                                   
  • Group/Genus: Trichovirus

  • Datasheet      Tech Support

Catalog: V148
Name: Apple Chlorotic Leaf Spot Virus
Acronym: ACLSV

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Apple Chlorotic Leaf Spot Virus


A rabbit polyclonal antibody for both capture and detection


DAS ELISA                                   


 The test reacted with all ACLSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.300 - >1.800 depending on the virus titer in the samples tested.


Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:90 -1:810.


There is no cross reaction with healthy plant  tissues such as apple or other host plants. Background is low on all of  the negative control wells.



Apple Chlorotic Leaf Spot Virus




 pear ring pattern mosaic virus (Cropley, 1969), apple latent virus type 1, plum pseudopox virus, quince stunt virus.




Main natural host plants: Malus platycarpa, Malus sylvestris cv. R12740-7A, Malus sylvestris cv. Spy, Malus sylvestris cv. Virginia Crab - stem pitting, Cydonia oblonga (Quince) C7/1, Pyronia veitchii - chlorosis and stunting, Pyrus communis - ring patterns, mosaic, Crataegus spp. - chlorotic ring spots, Prunus armeniaca - ring patterns, mosaic, Prunus spinosa - chlorotic ringspot patterns, Prunus persica, Prunus domestica and Prunus armeniaca.
Transmission: transmitted by mechanical inoculation; transmitted by grafting (possibly by root-grafts; Gilmer et al., 1971); not transmitted by seed.
Virus Infection:  Symptoms caused by viral infection include chlorotic leaf spots and stem pitting, line patterns and chlorotic rings, chlorotic spots and stem pitting, chlorosis and stunting, dark green mottle, bark split and pseudopox.
Geographical distribution: Probably distributed worldwide (and probably wherever apples are cultivated). Spreads in the Eastern Asian region, the Eurasian region, the North American region, and the Pacific region; Australia, China, and New Zealand.
Diagnostically susceptible host species and symptoms: Malus sylvestris cv. R12740-7A - asymmetric blotches and malformation of young leaves; M. platycarpa (Long Ashton clone) - irregular chlorotic rings and line patterns, small leaves shed prematurely, malformation; Chenopodium quinoa - large etched local lesions, becoming necrotic, systemic chlorotic spotting, mottling, ring and line patterns, chlorosis and necrosis; C. amaranticolor - chlorotic local lesions, surrounded by necrotic dots; Phaseolus vulgaris cvs Pinto, Bountiful, Kinghorn - purple-brown necrotic spots or rings (1-3 mm), not systemic..


Catalog Number Description Test Wells Ship Weight (Estimated)
V148-R1 ELISA Reagents, Alkaline Phosphatase 500 2 lb (1 kg)
V148-R2 ELISA Reagents, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V148-R3 ELISA Reagents, Alkaline Phosphatase 5000 12 lb (6 kg)
V148-R4 ELISA Reagents, Alkaline Phosphatase 100 1 lb (0.5 kg)
V148-K1 ELISA Kit, Alkaline Phosphatase 500 2 lb (1 kg)
V148-K2 ELISA Kit, Alkaline Phosphatase 1000 3 lb (1.5 kg)
V148-C1 Coating Antibody 500 2 lb (1 kg)
V148-C2 Coating Antibody 1000 2 lb (1 kg)
V148-C3 Coating Antibody 5000 2 lb (1 kg)
V148-D1 Detecting conjugate, Alkaline Phosphatase 500 2 lb (1 kg)
V148-D2 Detecting conjugate, Alkaline Phosphatase 1000 2 lb (1 kg)
V148-D3 Detecting conjugate, Alkaline Phosphatase 5000 2 lb (1 kg)
V148-P2 ELISA Control, Positive 18 1 lb (0.5 kg)
V148-N1 Negative control 18 1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 6 lb (3.0 kg)


1.  German, S., Candresse, T., Le Gall, O., Lanneau, M. and Dunez, J. (1992). J. gen. Virol. 73: 767.
2.  Lister, R.M. (1970). CMI/AAB Descr. Pl. Viruses No. 30, 4 pp.
3.  Lister, R.M. and Bar-Joseph, M. (1981). Closteroviruses. In: Handbook of Plant Virus Infections and Comparative Diagnosis, p. 809; ed. E. Kurstak. Elsevier/North-Holland Biomedical Press.

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