Product Details
Test Principle
This kit uses Competitive-ELISA as the method. It can detect Nitrofuran Furazolidone (AOZ) in samples, such as muscle, honey, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, AOZ in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AOZ antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of AOZ. The concentration of AOZ in the samples can be calculated by comparing the OD of the samples to the standard curve.
Product Specifications |
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Synonyms |
Metabolite 3-Amino-2-oxazolidinone |
Sample type |
muscle, liver, honey, milk, milk powder, egg powder, feed |
Reactivity |
Universal |
Sensitivity |
0.05 ppb (ng/mL) |
Reaction mode |
25℃, 45 min~15 min |
Detection limit |
Muscle, Liver, Honey, Milk, Egg ---0.1 ppb; Milk powder, Egg powder, Feed---0.1 ppb |
Cross reactivity |
Nitrofuran Furazolidone---100%, Furaltadone metabolite---<0.1%, Aminohydantoin Hydrochloride ---<0.1%, Nitrofurazone metabolite---<0.1%, |
Sample recovery rate |
Muscle, liver---85%±15%, Honey, Milk ---75%±15%, Milk powder, Egg powder, Feed---85%±25% |
Incubation Temperature |
- |
Incubation Time |
- |
Kit Contains |
|
Storage |
Store the kit at 2~8℃. Do not freeze any test kit components. |
Expiry date |
expiration date is on the packing box. |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Other materials required but not supplied
Instruments: Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g). |
Documents
Manual
Manual: AOZ(Nitrofuran Furazolidone) ELISA KitMSDS
MSDS: AOZ(Nitrofuran Furazolidone) ELISA KitReferences
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