REFERENCES

Features of Immunocapture RT-PCR Kits:

♥ Pre-coated PCR tubes for capturing virus particles which can be directly amplified by RT-PCR, and allowing entire assay to be performed in a single PCR tube.
♥ Saving time by eliminating the time-consuming sample RNA extraction procedure, and saving fund by not using expensive RNA extraction kit.
♥ Obtaining robust and consistent result every time running a RT-PCR by virtually getting rid of all PCR inhibitors through viral immunocapturing process.
♥ Cost-effective and user-friendly for RT-PCR tests of large number of samples.


Kit Components:

ACDcap Immunocapture Kit includes 1X8 PCR strips pre-coated with specific antibody, PCR sample extraction buffer, PCR plate washing buffer, positive control, negative control, and instruction.
ACDamp Immunocapture RT-PCR Kit includes 1X8 PCR strips pre-coated with specific antibody, PCR sample extraction buffer, PCR plate washing buffer, positive control, negative control, RT-PCR primers, and instruction.
ACDcom Complete Immunocapture RT-PCR Kit includes 1X8 PCR strips pre-coated with specific antibody, PCR sample extraction buffer, PCR plate washing buffer, positive control, negative control, RT-PCR primers, Master Mix, Reverse Transcriptase, Taq Polymerase, and instruction.


Brief Assay Procedure:

♠ Grind sample in PCR sample extraction buffer
♠ Dispense the ground sample into the tube of 1x8 PCR strip
♠ Prepare and add positive/negative controls into the control tube
♠ Incubate the plate for 2 hours at room temperature, or overnight at refrigerator
♠ Wash the PCR plate with PCR washing buffer
♠ Prepare and add the primers to master mix
♠ Add the master mix with enzymes into all of the PCR tubes
♠ Perform RT-PCR cycling