Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

Applicable For	Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.
Binding Capacity	400 µg
Culture Input	≤ 50 ml
Elution Volume	≥ 100 µl
Endotoxin Levels	≤ 0.025 EU/µg Plasmid DNA
Equipment	Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge
Format	Spin Column
Processing Time	20 min
Purity	Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
Size Range	Up to 200 kb
Yield	≤ 400 µg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)

Q1: What is the composition of the ZymoPURE Elution buffer?

Q2: Can the ZymoPURE Kits be used with other bacteria?

Q3: What type of vacuum pump do you recommend?

Q4: Are the ZymoPURE kits compatible with any commercially available vacuum manifold?

Q5: Can an in-house vacuum line be used with the EZ Vac Vacuum Manifold?

Q6: I ran out of ZymoPURE Wash 2. Can I substitute it with a homemade solution or Wash Buffer from another kit?

Q7: Is there a protocol for low-copy number plasmid DNA?

Q8: For low-copy plasmids, can I exceed the recommended volume of bacterial culture or use enriched growth media?

Q9: Can the ZymoPURE kits be used to isolate large plasmid constructs (BAC/PAC)?

“It went beyond my expectation. The protocol is very easy to follow. Very good DNA yields and the DNA was high quality”

– J. G., Yale University

“This kit it pretty great, midiprep DNA in about the time it takes to do a miniprep.”

– T. W., Northeastern University

“My student had an easy time with this kit also, and he had never done a DNA prep before.”

– K. G., Friedrich-Alexander University Erlangen-Nuremberg

“VERY fast protocol, Great color coding of the buffers, nanodrop curves looked perfectly fine, purity and quality perfect for my uses.”

– M. C., University of Pretoria

“I like this midiprep kit alot!! It can purify the plasmids with high quality in short period of time.”

– R. K., University of Alabama at Birmingham

“I have used the Promega and Qiagen kits, but even with following all the steps carefully, I have never got such high quality and concentrated DNA.”

– P. J., University of Miami

“This kit was absolutely amazing, quicker and easier to use than the Qiagen kit I have used previously”

– E. S., Queen Mary University of London

“With your kit, I got twice the amount of DNA that I usually get with Invitrogen’s midi kit, in half the time. Can’t beat that!”

– D. G., Immunology Consultants Laboratory

This is what I like about the kit

• high purity and yield.
• the protocol is short and easy to follow,
• there is an option of using of vacuum or centrifuge
• coloured buffers
• consumables are provided with the kit

– T. S., National Cancer Centre Singapore