ZymoPURE II Plasmid Maxiprep Kit

ZymoPURE Plasmid Midiprep Kits


  • Fastest: Simple 20 minute Maxipreps.
  • Highest Yield: Purify up to 1.2mg of plasmid DNA directly from a spin-column.
  • Ultra-Pure: EndoZero, vaccine grade, and transfection ready.


Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

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Technical Specifications

Applicable For Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.
Binding Capacity 1.2 mg
Culture Input ≤ 150 ml
Elution Volume ≥ 200 µl
Endotoxin Levels ≤ 0.025 EU/µg Plasmid DNA
Equipment Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.
Format Spin Column
Processing Time 20 min
Purity Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
Recommended Sample Volume ≤ 150 ml
Size Range Up to 200 kb
Yield ≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)

Product FAQ

Q1: How do I elute from the included Spin-Column?

Q2: What is the composition of the ZymoPURE Elution buffer?

Q3: Can the ZymoPURE Kits be used with other bacteria?

Q4: What type of vacuum pump do you recommend?

Q5: Are the ZymoPURE kits compatible with any commercially available vacuum manifold?

Q6: Can an in-house vacuum line be used with the EZ Vac Vacuum Manifold?

Q7: I ran out of ZymoPURE Wash 2. Can I substitute it with a homemade solution or Wash Buffer from another kit?

Q8: Is there a protocol for low-copy number plasmid DNA?

Q9: Can I process more than the recommended volume of bacterial culture or use enriched growth media?

Q10: Can the ZymoPURE kits be used to isolate large plasmid constructs (BAC/PAC)?



“Perfect! Finally, we got 600ug plasmid from 150ml E.coli culture in half an hour.”

– B. P., Los Angeles Biomedical Research Institute at Harbor-UCLA

“We were impressed by the high yield of plasmid DNA from this kit! From a 150 mL culture, I was able to isolate 0.912 mg DNA, in the same amount of time (and effort) I could get about half as much DNA from the comparable Qiagen kit.”

– C. S., Abbott Laboratories

“I was very satisfied with this product. It produced high DNA concentration eluting in 400 µl using the above mentioned cell line, which would generally only be obtainable with other kits eluting in much smaller volumes. This product is also miles ahead of other general Maxiprep kits in terms of time required for plasmid extraction.”

– J. P., University of Pretoria

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Kit Components

ZymoPURE II Plasmid Maxiprep Kit


Cat # Name Size
D4203 ZymoPURE II Plasmid Maxiprep Kit 20 Preps.
D4202 ZymoPURE II Plasmid Maxiprep Kit 10 Preps.