- Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels.
- Unique column design for low volume (≥ 10 µl) elution of ultra-pure, high-yield DNA.
- Eluted DNA is well-suited for use in endonuclease digestion, sequencing, labeling, PCR, etc.
|Applicable For||High-quality, purified DNA is ideal for sequencing ligation reactions, and any other sensitive downstream applications|
|Elution Volume||≥ 10 µl|
|Purity||High-quality, purified DNA is especially well-suited for sequencing and ligation reactions|
|Sample Source||Agarose gels containing large DNA fragments of interest|
|Sample storage||Eluted DNA can be used immediately or stored at ≤ -20°C|
|Size Range||50 bp to 200 kb|
|Yield||Up to 10 µg DNA|
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
– Ingrid M.
“The ADB buffer and higher temperature used melted the gel quicker than previously used kits thus making DNA recovery using this kit more efficient and generally quicker than previously used kits.”
– Kelly-Anne F.
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
– Thomas B (University of Tennessee, Knoxville)
“Protocols are very well written and Instructions easy to follow. Always had good results with your products.”
– Simin H. (University of California, Irvine)
“It’s a kit with a faster protocol and [has] a very good efficiency in quantity of DNA recovered. Also have a possibility to elute only in 6 µl of water. “
– Joao B, (Instituto de Tecnologia Química e Biológica)
“I had tried three other kits to do a certain recovery before this, I was getting bad yields, the elution volume required was quite large, the protocols were not as easy to follow and they took longer to complete. I liked everything about this kit, it is simple, efficient and quick. The protocol is easy to follow and the yield was 4-5 times better than what I got from other kits.”
– Wendy P. (University of Auckland)
“I did a direct comparison with the Qiagen kit (which we normally use) on duplicate samples. I found the yields to be the same, but the high concentration from Zymo resulting from the low elution volume was extremely helpful for a downstream ligation. That ligation also showed that the DNA products from both kits performed exactly the same (within the resolution of a standard gel). The elution concentration is probably the best aspect. The reduced number of buffers and steps is also nice, as are the low-bind column containers. I’ll probably be using this kit from now on.”
– Adam C. (University of Texas, Southwestern)
Zymoclean Large Fragment DNA Recovery