- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 10 µl ZymoBIOMICS DNase/RNase Free Water|
|Equipment||Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).|
|Processing Volume||Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2×108 bacterial, 2×107 yeast, or 2×106 mammalian cells)|
|Purity||High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8|
|Sample Source||Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.|
|Sample Storage||Eluted DNA should be stored at ≤ -20°C.|
|Size Range||Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.|
|Yield||Up to 5 µg total DNA can be eluted into ≥ 10 µl|
ZymoBIOMICS DNA Microprep Kit