
Highlights
- Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
- Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
Description
Technical Specifications
Equipment | Automated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually. |
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Purity | DNA and RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
Sample Source | Any cells, solid tissue, whole blood, biological fluids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), samples stored in DNA/RNA Shield™, etc. |
Size Range | Genomic DNA ≥40 kb and Total RNA ≥17 nt |
Yield | 20 µg DNA and RNA (binding capacity), ≥50 µl (elution volume) |
Citations
Reviews
“This kit was invaluable. I was able to get at least 1 µg of RNA and several hundred nanograms of DNA from each sample – all of it high quality. Much better than my previous experience with a different dual DNA/RNA isolation kit.”
– Julie P. (CU Anschutz Medical Campus)
“I usually recommend this kit for those who want to extract both DNA and RNA from a single sample. We have even had great quality and yield for single insect samples.”
– Robert B. (Harvard University)
“The kit is very economical for simultaneous extraction of high quality DNA and RNA from very small amount of biological samples. More particularly, RNA is of good quality that suits best for applications like next-generation sequencing (RNA-Seq).”
– Muhammad A. (Northwestern University)
“The kit is awesome! Besides saving a lot of time purifying gDNA and RNA at the same time, it also allows me to use cultures for other purposes since both can be extracted from the same sample.”
– Fernando B. (MIT)
“”Love this kit. I work with small mites and I get great results. I have tried other kits and none perform the same. Technical support is also great and they do their best to answer your questions and concerns.”
– Laura T. (USDA PPQ APHIS CPHIST)
Kit Components
Cat # | Name | Size |
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R1200-25 |
DNA/RNA Shield (2X Concentrate) | 25 ml |
R1200-125 |
DNA/RNA Shield (2X Concentrate) | 125 ml |
D4100-2-12 |
MagBinding Beads | 12 ml |
D4100-2-3 |
MagBinding Beads | 3 ml |
W1001-10 |
DNase/RNase-Free Water | 10 ml |
W1001-30 |
DNase/RNase-Free Water | 30 ml |
D7001-1-200 |
DNA/RNA Lysis Buffer | 200 ml |
D7001-1-50 |
DNA/RNA Lysis Buffer | 50 ml |
D7010-2-200 |
DNA/RNA Prep Buffer | 200 ml |
D7010-2-50 |
DNA/RNA Prep Buffer | 50 ml |
R2130-1-120 |
MagBead DNA/RNA Wash 1 | 120 ml |
R2130-1-30 |
MagBead DNA/RNA Wash 1 | 30 ml |
R2130-2-20 |
MagBead DNA/RNA Wash 2 | 20 ml |
R2130-2-80 |
MagBead DNA/RNA Wash 2 | 80 ml |
E1010-1-4 |
DNA Digestion Buffer | 4 mL |