The Quick-DNA FFPE Kit provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using the provided Proteinase K, heat, and then purify the DNA with the Zymo-Spin columns in the kit. DNA >50 bp or >500 bp can be selectively isolated by altering the lysis buffer conditions as described in the protocol. PCR inhibitors are effectively removed during the isolation procedure and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc.

Applicable For	All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Equipment	Microcentrifuge, heat block/bath (55°C and 90°C), isopropanol, beta-mercaptoethanol (optional).
Processing Volume	Tissue – ≤25 mg or (4) 20 µm thick with a surface area of 20 mm2
Purity	Typical A260/A280 ≥ 1.8
Sample Source	Tissue from paraffin block or tissue sections. Compatible with fresh/frozen tissue specimens
Sample Storage	Eluted DNA should be stored at ≤ -20°C.
Size Range	> 50 bp
Type	Total DNA
Yield	Up to 25 µg total DNA can be eluted into ≥ 50 µl.