CpG Methylase (M. Sssl)

In vitro methylation of DNA

Highlights

  • For complete, in vitro methylation of DNA for methylation analysis.
  • Methylation of chromatin DNA for DNA accessibility studies.
  • Inhibition of endonucleases with overlapping CpG sequence recognition and
    [3H]-labeling of DNA.


Description

CpG Methylase completely methylates all cytosines in double-stranded, non-methylated, and hemimethylated DNA in a 5’…CpG…3′ context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis. This product is supplied with 10X Reaction Buffer and S-adenosylmethionine cofactor.

Technical Specifications

Additional Information Enzyme Commission Number (EC 2.1.1.37)
Assay Condition CpG Methylase in 1X CpG Reaction Buffer w/ 600 µM SAM. Incubate reaction mixtures at 30C (Performing the Methylase reaction at 30°C rather than 37°C promotes optimal reaction kinetics).
Heat Inactivation 65°C for 20 minutes
Source Recombinant methylase is isolated from E. coli expressing the methyltransferase gene from Spiroplasma sp. strain MQ1.
Storage Store at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles of reagents. Prolonged storage is at = -70°C.
Unit Definition One unit (U) is the amount of enzyme required to protect 1µg of ? DNA from cleavage by BstUI restriction endonuclease in a total reaction volume of 20 µl for 1 hour at 37°C.

Citations

Kit Components

CpG Methylase (M. Sssl)

Cat # Name Size
E2010 CpG Methylase (M. Sssl) 200 U
E2011 CpG Methylase (M. Sssl) 400 U

Documents

Protocol: 
SDS (MSDS):