Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IHC-P, IHC
- Reactivity: Human
- Predicted Reactivity:
-
Datasheet
Tech Support
IMAGES
Flow cytometric analysis of Tryptophanyl tRNA synthetase / WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54661R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Human lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody, Unconjugated (bsm-54661R) at 1:200 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within C-terminal human Tryptophanyl tRNA synthetase/WRS. |
| IMMUNOGEN RANGE |
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| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
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| SUBCELLULAR LOCATION |
Cytoplasm |
| SYNONYMS |
Gamma 2 antibody, GAMMA-2 antibody, Gamma2 antibody, hWRS antibody, IFI 53 antibody, IFI53 antibody, IFP 53 antibody, IFP53 antibody, Interferon induced protein 53 antibody, Interferon-induced protein 53 antibody, SYWC_HUMAN antibody, T2-TrpRS antibody, TrpRS antibody, Tryptophan tRNA ligase, cytoplasmic antibody, Tryptophan tRNA ligase 1 cytoplasmic antibody, Tryptophan tRNA ligase antibody, Tryptophan--tRNA ligase antibody, Tryptophanyl tRNA synthetase antibody, Tryptophanyl tRNA synthetase cytoplasmic antibody, WARS antibody, WARS protein antibody, WRS antibody |
| BACKGROUND |
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| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400), IHC() |
