TLR7 Polyclonal Antibody, FITC Conjugated
- Size: 100ul
- Application: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity: Dog, Cow, Pig, Horse
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Datasheet
Tech Support
IMAGES
Paraformaldehyde-fixed, paraffin embedded mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with TLR7 Polyclonal Antibody, FITC Conjugated at 1:400 for 90 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with TLR7 Polyclonal Antibody, FITC Conjugated at 1:400 for 90 minutes at 37°C.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
TLR7 Polyclonal Antibody, FITC Conjugated |
|
CONJUGATION |
FITC |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthetic peptide derived from human TLR7 |
| IMMUNOGEN RANGE |
461-560/1049 |
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at 4°C for 12 months. |
TARGET |
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|---|---|
| GENE ID |
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| SUBCELLULAR LOCATION |
Cytoplasm, Cell membrane |
| SYNONYMS |
TLR7-like; Toll-like receptor 7; TLR7; UNQ248/PRO285 |
| BACKGROUND |
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). |
| APPLICATION DILUTION |
FCM(1:20-100), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200) |
