Thyroglobulin Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P, IHC
- Reactivity: Human
- Predicted Reactivity:
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Datasheet
Tech Support
IMAGES
Paraformaldehyde-fixed, paraffin embedded Human thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Thyroglobulin Monoclonal Antibody, Unconjugated (bsm-54657R) at 1:50 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Thyroglobulin Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Recombinant protein within C-terminal human Thyroglobulin. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
Secreted |
| SYNONYMS |
AITD 3 antibody, AITD3 antibody, hTG antibody, TDH3 antibody, Tg antibody, TGN antibody, THYG_HUMAN antibody, Thyroglobulin antibody |
| BACKGROUND |
Thyroglobulin is the glycoprotein precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin is obtained from the thyroid gland and exhibits the general properties of the globulins. The human thyroglobulin (hTG) is a high molecular weight glycoprotein (605 kDa) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are, in large part, due to autoimmune origin, and anti thyroglobulin autoantibodies were the first factor to be discovered. Anti hTG is found in all thyroid autoimmune diseases (Hashimoto's thyroiditis, Graves' diseases), with the highest level observed in Hashimoto's thyroiditis. Anti hTG is also characteristic of thyroid cancer, and its determination can be used for the follow up of cancer patients. |
| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400), IHC() |