SAA4 Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IHC-P
- Reactivity: Human
- Predicted Reactivity:
-
Datasheet
Tech Support
IMAGES
Flow cytometric analysis of SAA4 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54660R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SAA4 Monoclonal Antibody, Unconjugated (bsm-54660R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SAA4 Monoclonal Antibody, Unconjugated (bsm-54660R) at 1:50 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
SAA4 Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic peptide within C-terminal Human SAA4. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
|
|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Secreted |
| SYNONYMS |
Amyloid A, serum, 4 antibody, C SAA antibody, C-SAA antibody, Constitutively expressed serum amyloid A protein antibody, CSAA antibody, SAA 4 antibody, Saa4 antibody, SAA4 protein antibody, SAA4_HUMAN antibody, Serum amyloid A 4 protein antibody, Serum amyloid A-4 protein antibody, Serum amyloid A4 constitutive antibody |
| BACKGROUND |
SAA4 is a constitutively expressed protein belonging to the SAA family. It is a major acute phase reactant and an apolipoprotein of the HDL complex. The serum amyloid A (SAA) superfamily comprises a number of differentially expressed genes with a high degree of homology in mammalian species. SAA4, an apolipoprotein constitutively expressed only in humans and mice, is associated almost entirely with lipoproteins of the high density range. Its physiological function is unknown and its serum concentration has no relationship with those of other major apolipoproteins. The presence of SAA4 mRNA and protein in macrophage derived foam cells of coronary and carotid arteries suggested a specific role of human SAA4 during inflammation including atherosclerosis. |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400) |